摘要
目的 建立虎杖药材的HPLC-DAD-ELSD指纹图谱,对10个不同产地的虎杖药材进行质量评价。方法 使用Luna C_（18）（2）色谱柱（250mm×4.6mm,5μm）,乙腈-水为流动相进行梯度洗脱,体积流量1.0mL/min,柱温35℃,检测波长254 nm,进样量10μL。蒸发光检测器漂移管温度109℃,载气体积流量3.0 L/min。结果 通过10批虎杖药材建立了指纹图谱的共有模式,其中HPLC-DAD指纹图谱共有峰19个,相似度为0.938-0.993;HPLC-ELSD指纹图谱共有峰14个,相似度为0.905-0.999。确认了虎杖苷、白藜芦醇、大黄素-8-O-β-D-葡萄糖苷、大黄素甲醚-8-O-β-D-葡萄糖苷、大黄素和大黄素甲醚。结论 方法 准确、稳定,为客观评价虎杖药材的质量提供了科学依据。
Objective To establish the chromatography fingerprint of Polygoni Cuspidati Rhizoma et Radix with hyphenated technique of HPLC-DAD-ELSD and to evaluate Polygoni Cuspidati Rhizoma et Radix from 10 different origins.Methods Luna C_（18）（2） column（250 mm × 4.6 mm,5 μm） was used.Mobile phase was acetonitrile-H_2O;Flow speed was 1.0 mL/min;Temperature of column was set at 35 ℃;Detective wavelength was at 254 nm;Injection volume was 10 μL.The temperature of drift tube was 109℃ and the flow speed was 3.0 L/min.Results The chromatography fingerprint of Polygoni Cuspidati Rhizoma et Radix from 10 different origins was established.In the chromatography fingerprint with HPLC-DAD of Polygoni Cuspidati Rhizoma et Radix,19 common peaks were demarcated and the similarities of Polygoni Cuspidati Rhizoma et Radix were between 0.938—0.993.In the chromatography fingerprint with HPLC-ELSD of Polygoni Cuspidati Rhizoma et Radix,14 common peaks were demarcated and the similarities of Polygoni Cuspidati Rhizoma et Radix were between 0.905—0.999.Polydatin,resveratrol,emodin-8-O-β-D-glucoside,physcion-8-O-β-D-glucoside,emodin,and physcion were identified.Conclusion The method is accurate and stable,which can be used as the evidence for the quality evaluation of Polygoni Cuspidati Rhizoma et Radix.
出处
《中草药》
CAS
CSCD
北大核心
2015年第12期1830-1835,共6页
Chinese Traditional and Herbal Drugs
基金
吉林省科技厅课题"虎杖有效部位治疗高脂型糖尿病的研究"(201307026YY)
