摘要
探讨一个准确有效的测定乳制品中VK1含量的方法。样品经KOH皂化、石油醚萃取后,用C18分析,以甲醇为流动相,在吸收波长248 nm下检测VK1。结果表明：方法线性范围为0～4.0μg/m L,线性相关系数为0.999 6,加标回收率≥90.0%,相对标准偏差≤2.0%;方法精密度和回收率均优于GB 5413.10-2010且可同时检测VA,VD,VE,方法快速、准确、有效。
The aim of this study was to establish an accurate and effective method for determining Vitamin K1 in dairy products. After extraction,the samples were analyzed by C18,with methanol as mobile phase. The absorption wavelength of 248 nm was chosen to detect vitamin K1. The results showed that calibration curves were linear between0 ～ 4. 0μg / m L,and the correlation coefficients were 0. 9996. The recoveries of the vitamin K1 in the dairy products were more than 90. 0% with relative standard deviations less than 2. 0%. This method is better than the national standard method GB 5413. 10-2010 in terms of precision and recovery. In addition,it can simultaneously determine the level of vitamin A,vitamin D and vitamin E. This is an effective and accurate method for determination of vitamin K1.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2014年第12期161-164,共4页
Food and Fermentation Industries
关键词
乳制品
VK1
改进
高效液相色谱法
dairy products
vitamin K1
improvement
high performance liquid chromatography
