摘要
目的:观察连花清瘟胶囊(LHQW)对汽车尾气暴露致小鼠肺组织病理损伤及炎症因子表达的干预作用,探讨连花清瘟胶囊的作用机制。方法:75只健康SPF级雄性昆明种小鼠随机分为LHQW 2,4,8 g·kg-1剂量组,另设溶媒组、模型组,采用汽车尾气染毒柜染毒,同时,每天灌胃给药1次,连续14 d,末次染毒24 h后取肝素抗凝血,流式细胞仪检测白介素-4(IL-4),白介素-6(IL-6)的阳性表达率,取血清检测白介素-12(IL-12)含量,取肺组织苏木精-伊红(HE)染色观察病理改变,Western blot和实时定量PCR法分别检测肺组织里肿瘤坏死因子-α(TNF-α),白介素-1β(IL-1β),IL-4,IL-6,IL-12,IL-13的蛋白及其mRNA表达。结果:HE染色后光镜下,与溶媒组比较,模型组可见支气管上皮肿胀,上皮细胞增生、坏死、脱落,肺泡内可见出血及渗出,血管周围可见水肿;与模型组比较,LHQW 4,8 g·kg-1组小鼠肺组织病理损伤明显减轻;LHQW 2,4,8 g·kg-1组血中IL-6阳性表达率分别为20.1%,17.6%,11.4%,远低于模型组的32.9%;与溶媒组比较,模型组肺组织里TNF-α,IL-1β,IL-4,IL-6,IL-12,IL-13蛋白和mRNA的表达均显著升高(P<0.01);与模型组比较,LHQW 2,4,8 g·kg-1组肺组织中的TNF-α,IL-1β,IL-4,IL-6,IL-12,IL-13蛋白和mRNA均有不同程度的降低。结论:连花清瘟胶囊通过降低血液里炎性因子的含量,降低肺组织炎性因子的蛋白表达和mRNA的表达,从而改善汽车尾气造成肺组织的病理损伤。
Objective： To observe the protective effects and mechanism of Lianhua Qingwen（ LHQW）capsule on lung tissue injury and expression of inflammatory cytokines in mice exposed to automobile exhaust.Method： Seventy-five healthy SPF male KM mice were randomly divided into 5 groups： normal group,model group,LHQW 2,4,8 g·kg- 1groups,and exposed to automobile exhaust in homemade cabinet in a noncontinuous way. The experimental animals were euthanatized 24 h later from the last exposure. The blood was used to detect the positive expression ratio of interleukin（ IL）-4,IL-6 with flow cytometry. The serum of other animals was adopted to detect the content of IL-12 with ELISA. The pathological changes in lung tissue were determined with hematoxylin and eosin（ HE） staining. Western-blot and real-time quantitative PCR were used to analyze the protein and mRNA expression of tumor necrosis factor-α（ TNF-α）,IL-1β,IL-4,IL-6,IL-12 and IL-13 in lung tissue. Result： The visible bronchial epithelium swelling, epithelial cell proliferation, necrosis, alveolar hemorrhage and exudation,perivascular edema could be seen in model group,the injury of lung tissue in LHQW 4,8 g·kg- 1group was significantly reduced. The positive expression ratio of IL-6 was 20. 1%,17. 6%,11. 4% in mice blood of LHQW 2,4,8 g·kg- 1group respectively,obviously lower than 32. 9% in model group. Compared with normal group, the protein and mRNA expression of TNF-α, IL-1β, IL-4, IL-6, IL-12, IL-13 were significantly increased in the lung tissue of model group,and LHQW at the dose of 2,4,8 g·kg- 1could inhibit the expression at different extent. Conclusion： LHQW capsule could reduce the lung damage in mice exposed to automobile exhaust through reducing the content of inflammatory cytokines in the blood and lung tissues.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第13期139-143,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省中医药管理局科研计划项目(2014227)
关键词
连花清瘟
汽车尾气
病理损伤
炎症因子
Lianhua Qingwen capsule
automobile exhaust
tissue injury
inflammatory cytokines
