摘要
目的比较胶体金免疫层析法和酶联免疫吸附试验(ELISA)检测血清抗环瓜氨酸肽(CCP)抗体结果差异性。方法采用胶体金免疫层析法和ELISA法同步检测110例RA患者和110例健康体检者的抗CCP抗体。结果 2种方法经配对χ2检验,差异有统计学意义(χ2=174.354,P<0.01);Kappa值评价(K=0.890,P<0.01)显示2种检测方法具有高度一致性。ELISA法灵敏度和特异度分别为83.64%和92.73%,均优于胶体金免疫层析法。结论 2种方法检测血清抗CCP抗体存在差异,ELISA法的灵敏度和特异度均较高,优于胶体金免疫层析法。
Objective To evaluate the diagnostic values of enzyme‐linked immunosorbent assay （ELISA ） and colloidal gold im‐munochromatographic assay in detecting serum anti‐cyclic citrullinated peptide（anti‐CCP） antibody .Methods Colloidal gold immu‐nochromatographic assay and ELISA were used to detect serum anti‐CCP antibody in 110 RA patients and 110 healthy cases . Results The data were analysed with the paired Chi‐Square Test （χ2 = 174 .354 ,P 〈 0 .01） ,the difference was significant .The K ap pa value was 0 .890（P〈 0 .01） ,indicating that the coincidence between the two kinds of methods was good .The sensitivity , speciality of ELISA were 83 .64% and 92 .73% respectively .Conclusion In detecting serum anti‐CCP Antibody ,there was dife‐rence between colloidal gold immunochromatographic assay and ELISA according to statistics analysis .The sensitivity and speciality of ELISA were higher than those of colloidal gold immunochromatographic assay .
出处
《国际检验医学杂志》
CAS
2015年第11期1528-1529,共2页
International Journal of Laboratory Medicine
基金
甘肃省卫生行业科研计划管理资助项目(GWGL2013-1)
关键词
酶联免疫吸附试验
抗环瓜氨酸肽抗体
类风湿关节炎
enzyme-linked immunosorbent assay
anti-cyclic citrullinated peptide antibody
rheumatoid arthritis
