摘要
目的探讨白芍总苷(TGP)对系统性红斑狼疮(SLE)患者外周血B细胞内Toll样受体9(TLR9)表达的影响。方法选取SLE患者60例和正常健康人30例,分离外周血单个核细胞(PBMC),每份标本均分为4组体外培养,即空白对照组、胞嘧啶-磷酸-鸟嘌呤-寡聚脱氧核苷酸(Cp G-ODN)组、Cp G-ODN+TGP组和TGP组。空白对照组仅加入RPMI-1640完全培养液,其他3组分别加入Cp G-ODN(ODN终浓度1μmol·L-1)、Cp G-ODN+TPG(TPG最适终浓度为1×10-4mol·L-1)、TPG。各组均培养48 h后提取细胞,应用流式细胞仪检测各组B细胞内TLR9表达。结果 1正常健康人:Cp G-ODN组和空白对照组TLR9的表达率分别为(9.10±2.12)%,(4.96±2.11)%(P<0.01);2SLE患者:Cp G-ODN组的TLR9表达率(14.86±3.42)%,显著高于空白对照组的(9.20±3.43)%(P<0.01),Cp G-ODN+TGP组的TLR9表达率(11.95±3.63)%,低于Cp G-ODN组(P<0.05);在SLE轻度活动患者中,Cp G-ODN组和空白对照组TLR9的表达率分别为(10.74±3.17)%,(5.19±2.05)%(P<0.05);在SLE中重度活动患者中,Cp G-ODN组和空白对照组TLR9的表达率分别为(16.51±1.72)%,(10.80±2.37)%(P<0.01),Cp G-ODN+TGP组TLR9的表达率(13.59±2.58)%,低于Cp G-ODN组(P<0.01)。结论 TGP可以拮抗Cp G-ODN对B细胞内TLR9表达的上调作用。
Objective To investigate the effect of total glucosides of paeony （ TGP ） on the expression of Toll-like receptor 9 （TLR9） in peripheral blood B lymphocytes of the patients with systemic lupus erythematosus （SLE）. Methods Sixty SLE patients and thirty healthy volunteers were enrolled, peripheral blood mononuclear cells （ PBMC） were isolated from blood samples and divided into 4 groups, which were incubated with CpG-ODN（final concentration was 1 μmol·L^-1）, CpG-ODN＋TGP （ TPG final concentration was 1×10^-4 mol·L^-1 ） ,TGP and RPMI medium （ as the blank control group） for 48 hours, respectively. FLA （ Flow cytometry analysis） was used to detect the expression of TLR9 on peripheral blood B lymphocytes after incubated. Results ①In the health people, TLR9 expression in the group of CpG-ODN was（9. 10±2. 12） %, which was higher than the blank control group（4. 96±2. 11） % （P〈0. 01）.②In the SLE patients, the TLR9 expression in the group of CpG-ODN was（14. 86±3. 42）% , which was significantly higher than the blank control group（9. 20±3. 43） %（P〈0. 01）. The TLR9 expression in the group of CpG-ODN＋TGP was （11. 95±3. 63）%, which was lower than that in the group of CpG-ODN （P〈0. 05）. The TLR9 expression in the group of CpG-ODN with lightly active degree SLE patients was （10. 74±3. 17）%, which was higher than the blank control group（5. 19±2. 05） % （P〈0. 01）. For SLE from the moderately to vigorously active degree, the expression of TLR9 in the group of CpG-ODN（16. 51±1. 72） % was higher than the blank control group（10. 80±2. 37） %（P〈0. 01）, but that in the group of CpG-ODN＋TGP （13. 59±2. 58） % was lower than the group of CpG-ODN （P〈0. 01）. Conclusion Our data indicate that TGP antagonize upregulation effect of CpG-ODN on the expression of TLR9 in peripheral blood B lymphocytes.
出处
《医药导报》
CAS
2015年第5期589-593,共5页
Herald of Medicine
基金
蚌埠医学院校自然科学项目(Byky1352)
关键词
白芍总苷
红斑狼疮
系统性
寡聚脱氧核苷酸
TOLL样受体
Total glucosides of paeony
Lupus erythematosus,systemic
Oligodeoxynucleotides
Toll-like receptor
