摘要
目的 应用实时荧光PCR技术检测不同剂量^60Co γ射线照射后不同时间永生化淋巴细胞系AHH-1中S100A4基因的表达变化情况,探讨其基因表达变化的剂量和时间效应关系.方法 永生化淋巴细胞系AHH-1接受0、1、3、5、8、10、15、18 Gy^60Coγ射线照射后4、8、12、24、48和72 h,利用反转录聚合酶链反应（PCR）以及实时荧光PCR技术检测细胞S100A4基因表达水平,分析各时间点其基因的相对表达水平与不同照射剂量之间的关系.结果 照射后各时间点,一定剂量范围内,AHH-1中S100 A4基因的表达水平上调,与照射剂量存在着一定的剂量-效应关系（R^2 =0.79～0.93,P＜0.05）;在一定时间范围内,AHH-1中S100A4基因表达水平的上调受照射后时间的影响（F=8.91,P＜0.01）.结论 在一定剂量范围内,辐射诱导的S100A4基因表达在转录水平的变化检测便捷,具有较好的剂量-效应关系,初步具备作为生物剂量计的基本条件.
Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation.Methods AHH-1 cells was exposed to different doses（0,1,3,5,8,10,15 and 18 Gy）of ^60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4,8,12,24,48 and 72 h after irradiation.Results Within the range of applied doses,the level of S100A4 gene expression was upregulated with a good dose-response （R^2 =0.79-0.93,P 〈 0.05） and had obvious difference at different time points （F =8.91,P 〈 0.01）.Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation,and hence is applicable as a dosimeter.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2015年第4期245-247,302,共4页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金（81172593）
