摘要
Objective: To explore the expression of hypoxia inducible factor-1α (HIF-1α) and the correlation between HIF-1α and apoptosis after traumatic brain injury. Methods: Using experimental traumatic brain injury in the rats, the expression of HIF-1α was studied by immunohisto-chemistry in cerebral tissue, apoptotic cell death was evaluated with TUNEL (transferase-mediated X-dUTP nick end labeling), and double-labeled immunohistochemistry and TUNEL methods were used to investigate the relationship between HIF-1α and apoptosis. Results: There was remarkable difference in the expression of HIF-1α between the experimental groups and the control groups (P< 0.01), in the experimental groups, the expression of HIF-1α at 48 hours was highest; the evidence of apoptotic cell death after experimental traumatic brain injury was found by TUNEL; the apoptotic percentage increased or decreased according to the changes of the positive expression of HIF-1α (r= 0.99). Conclusions: The results suggest that secondary brain ischemia plays a crucial role in apoptotic cell death after traumatic brain injury; HIF-1α can prompt apoptotic cell death after experimental traumatic brain injury.
基金
JiangsuProvinceTechnology AssociationFoundation (No .DJ995 0 1)
