摘要
以籽瓜为试材,分别以芦丁和葫芦素B为标准品,采用亚硝酸钠-硝酸铝显色法和香草醛-硫酸比色法分别测定籽瓜中总黄酮和总皂苷含量,为籽瓜质量控制提供科学依据。结果表明:总黄酮线性方程为Y=0.0059C-0.0081,R2=0.9994;总皂苷线性方程为Y=0.005C-0.0335,R2=0.9991。数据表明这2种测定方法准确,且重复性好。籽瓜不同部位的总黄酮和总皂苷含量分别在这2种方法下随生长时期变化而变化,根据这些数据可以为籽瓜的综合开发利用提供参考依据。
Taking Citrullus lanatus as test material,the contents of total flavonoids and total saponins fromCitrullus lanatus was determined by sodium nitrite-aluminum nitrate chromomeric method and vanillin-sulfuric acid method,which provide a scientific basis for the quality control of Citrullus lanatus.To determinate the contents of total flavonoids and total saponins fromCitrullus lanatus,the rutin and cucurbitacin B were used as standard product.The results showed that,the linear equations of total flavonoids and total saponins determination were respectively Y=0.0059C-0.0081,R2=0.9994 and A=0.005C-0.0335,R2=0.9991.Data showed that the contents of total flavonoids and total saponin in different parts of Citrullus lanatus with growth period changes.The method was accurate and reproducible,which provided a theoretical basis for the comprehensive development and utilization of Citrullus lanatus.
出处
《北方园艺》
CAS
北大核心
2015年第9期109-112,共4页
Northern Horticulture
基金
兵团科技援疆计划资助项目(2012AB013)
关键词
籽瓜
不同生育期
不同部位
总黄酮
总皂苷
分光光度法
Citrullus lanatus
different growth phases
different parts
total flavonoids
total saponins
spectrophotometricmethod
