摘要
目的:研究姜黄素对急性心肌梗死模型大鼠的保护作用并探讨其机制。方法:结扎冠状动脉前降支以复制大鼠急性心肌梗死模型。100只SD大鼠分为假手术(ig,等容生理盐水)组、模型(ig,等容生理盐水)组、溶剂对照[ip,6%乙醇-聚乙二醇400溶液,2.5 ml/kg]组、培哚普利(ig,3 mg/kg)组与姜黄素高、中、低剂量(ip,200、100、50 mg/kg)组,复制模型24 h后开始给药,每天1次,连续4周。测定大鼠血液动力学指标[左室舒张末压(LVEDP)、左室收缩末压(LVSP)、左室内压最大上升/下降速率(±dp/dtmax)];计算左、右心室质量指数(LVMI、RVMI);计算心肌梗死面积(IS),测定心肌胶原面积并计算心肌胶原容积分数(CVF);采用免疫组化法测定大鼠心肌基质金属蛋白酶(MMP)-2的表达。结果:与假手术组比较,模型组大鼠LVSP、±dp/dtmax降低、LVEDP、LVMI、RVMI升高,IS增加,CVF升高,MMP-2表达增强,差异有统计学意义(P<0.01)。与模型组比较,姜黄素高、中、低剂量组大鼠LVSP、±dp/dtmax升高,LVMI、RVMI降低,CVF降低,MMP-2表达减弱;姜黄素高、中剂量组大鼠LVEDP降低,差异有统计学意义(P<0.01或P<0.05)。结论:姜黄素对急性心肌梗死模型大鼠具有一定保护作用,其机制可能与减轻心肌肥厚、减少胶原沉积、降低MMP-2的表达有关。
OBJECTIVE： To investigate the protective effect of curctunin on rats with acute myocardial infarction （AMI）, and discuss the protection mechanism. METHODS： Anterior descending coronary arteries were ligated to establish rat models of AMI. 100 SD rats were randomly divided into sham-operation group （ig, constant volume normal saline）, model group （ig, constant vol- ume normal saline）, solvent control group [ip, 6% alcohol-polyethylene glycol 400 solution, 2.5 ml/kg, perindopril group （ig, 3 mg/kg） and curcumin high, medium and low dose groups （ip, 200, 100 and 50 mg/kg）. They were given the drug 24 h after the establishment of models, once a day for 4 weeks. Haemodynamic indexes [left ventricular end-diastolic pressure （LVEDP）], left ventricular end-systolic pressure （LVSP） and maximtma rate of left ventricular pressure rise/decline （ ± dp/dtmax）] were determined; left and right ventricular mass indexes （LVMI and RVMI） and myocardial infarction areas （IS） were calculated; the areas of myo- cardial collagen were determined and the collagen volume fractions （CVF） were calculated; and immunohistochemical method was adopted to detect the expression of matrix metalloproteinase-2 （MMP-2） in rats. RESULTS： Compared with sham-operation group, the LVSP and ＋ dp/dt^x of rates in model group were decreased; LVEDP, LVMI, RVMI, IS, CVF and expression of MMP-2 were increased, with significant difference （P〈0.01）. Compared with model group, the LVSP and ± dp/dtmax of rats in curcumin high, medium and low dose groups were increased, and LVMI, RVMI, CVF and expression of MMP-2 were decreased; the LVEDP of rats in curcumin high and medium dose groups were decreased, with significant difference （P〈0.01 or P〈0.05）. CON- CLUSIONS： Curcumin has certain protective effect on model rats with AMI. The mechanism may be related to the reduction of myocardial hypertrophy, collagen deposition and the expression of MMP-2.
出处
《中国药房》
CAS
北大核心
2015年第13期1782-1785,共4页
China Pharmacy
