摘要
实验通过PCR-SSCP技术,对中国美利奴羊(新疆型)KRT27基因的多态性进行了检测,并对不同基因型与中国美利奴羊(新疆型)羊毛细度性状间的关联性进行了分析。结果表明:外显子5的一段长度为122 bp的扩增产物经SSCP分析后出现了两种基因型AA和AB,存在两种等位基因A和B;AA基因型和AB基因型在中国美利奴羊(新疆型)中的频率分布分别为0.86和0.14,等位基因A、B的基因频率分别为0.93和0.07,表明A等位基因为优势等位基因;所研究群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05)。不同基因型的个体间羊毛细度性状没有显著差异(P>0.05)。
In this study, PCR-SSCP analysis was used to identify genetic variation in KR/27 in Chinese Merino （Xinjiang type）, and the relationship with fineness traits were analyzed.The results showed that a PCR product of 122 bp corresponding to partial exon 5 had two genotypes, and the frequencies of the genotype AA, AB and allele A, B in Chinese Merino sheep （Xinjiang type） were 0.86,0.14 and 0.93,0.07 respectively, and the A allele was dominant allele. The polymorphic locus of KRT27 gene was at Hardy-Weinberg equilibrium（P〉0.05 ）in the breed. The individuals of genotype AA, AB had no significant difference in the fineness traits（P〉0.05 ）.
出处
《中国草食动物科学》
CAS
2015年第2期1-4,共4页
China Herbivore Science
基金
现代农业产业技术体系(CARS-40)
农业科技成果转化资金项目(2012GB2G400493)
紧缺人才专业大学生创新项目(jqrcp32010021)
