摘要
目的 研究MTB早期分泌靶抗原6/培养滤液蛋白10（ESAT-6/CFP-10）抗原对人单核细胞的活化T细胞核因子5（NFAT5）表达的影响.方法 收集解放军第三○九医院收治的活动性肺结核患者15例,男9例,女6例,年龄33 ～42岁,平均37岁.健康人来自解放军第三○九医院健康体检中心,共15名,男9名,女6名,年龄37 ～ 42岁,平均40岁.抽取患者和健康体检者的外周血,分离单核细胞,分别用H37Rv全菌裂解液和ESAT-6/CFP-10抗原多肽刺激,提取细胞总RNA,荧光定量PCR方法检测NFAT5 mRNA的表达,采用配对t检验分析刺激前后单核细胞NFAT5mRNA的表达差异.结果 活动性结核患者刺激前单核细胞NFAT5 mRNA的相对表达量为（0.23±0.15）,EAST-6/CFP-10抗原多肽刺激后为（0.18±0.12）,表达显著下调（t=2.591,P＜0.05）,而全菌裂解液刺激后表达量（0.28±0.25）没有显著变化（t=1.142,P＞0.05）.健康人刺激前单核细胞NFAT5 mRNA的相对表达量为（0.21 ±0.09）,EAST-6/CFP-10抗原多肽刺激后为（0.17±0.09）,表达显著下调（t=2.828,P＜0.05）,而全菌裂解液刺激后表达量（0.20±0.12）没有显著变化（t=0.452,P＞0.05）.结论 MTB分泌的ESAT-6/CFP-10抗原多肽能够抑制人单核细胞中NFAT5的表达.
Objective To study the effect of Mycobacterium tuberculosis antigens early secretory antigen target 6/culture filtrate protein 10 （ESAT-6/CFP-10） on nuclear factor of activated T-cells 5 （NFAT5） mRNA in monocytes from human peripheral blood.Methods Fifteen patients with active pulmonary tuberculosis （age range：33-42 y,mean：37 y,male/female：9/6） were recruited from the Institute of Tuberculosis in 309 Hospital,and 15 healthy subjects （age range：37-42 y,mean：40 y,male/female：9/6） were recruited from Medical Examination Center of the hospital.Monocytes were isolated from peripheral blood and stimulated with whole cell lysate of H37Rv strain and peptide pool of ESAT-6/CFP-10.Fluorescence quantitative PCR was used to detect the mRNA expression of NFAT5,and differences of NFAT5 mRNA between unstimulated and stimulated monocytes were analyzed by paired t test statistic method.Results The expression of NFAT5 mRNA in monocytes from patients with active pulmonary tuberculosis was significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes （0.18 ±0.12 vs.0.23 ±0.15） （t =2.591,P 〈0.05）.Similarly,the expression of NFAT5 mRNA in monocytes from healthy subjects was also significantly decreased when stimulated with peptide pool of ESAT-6/CFP-10 compared with unstimulated monocytes （0.17 ±0.09 vs 0.21 ±0.09） （t =2.828,P 〈 0.05）.However,the expression of NFAT5 mRNA in monocytes stimulated with whole cell lysate was not significantly changed （t =1.142,P 〉0.05）,which was 0.28 ±0.25 and 0.23 ±0.15 in stimulated and unstimulated monocytes,respectively.Similarly,the expression of NFAT5 mRNA in monocytes from healthy subjects was also not significantly changed when stimulated with whole cell lysate （t =0.451 9,P 〉0.05）,which was 0.20 ± 0.12 and 0.21 ± 0.09 in stimulated and unstimulated monocytes,respectively.Conclusion The MTB antigen ESAT-6/CFP-10 can inhibit NFAT5 expression in human monocytes in vitro.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2015年第4期290-293,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家自然科学基金青年基金（81101219）
关键词
分枝杆菌
结核
抗原
单核细胞
活化T细胞核因子5
Mycobacterium tuberculosis
Antigens
Monocytes
Nuclear factor of activated T-cells 5
