摘要
背景:为获得更加理想的人工骨材料,前期实验制备了纳米羟基磷灰石/丝素蛋白复合人工骨材料。目的:观察纳米羟基磷灰石/丝素蛋白复合人工骨材料修复骨缺损的效果。方法:将30只健康成年家兔随机均分为两组,制备单侧股骨中远段骨缺损模型,实验组于骨缺损处植入纳米羟基磷灰石/丝素蛋白复合人工骨材料,对照组于骨缺损处植入单纯羟基磷灰石,植入后4,8,12周取实验侧股骨,进行大体、X射线摄片、生物力学检测及组织学检查。结果与结论:随着时间的增长,两组生物力学强度逐渐增大,实验组术后不同时间点的生物力学强度均大于对照组(P<0.05)。术后12周时,实验组植骨区表面连续且光滑,色泽正常,与周围骨质无明显区别,人工骨材料完全降解吸收、被新生骨质替代,骨缺损区已完全愈合;对照组植骨区表面不光滑,表面骨皮质连续,与周围骨质界限不清,人工骨材料降解不完全,骨缺损处由骨痂连接,材料与骨质结合较紧密,骨缺损部分修复。结果表明纳米羟基磷灰石/丝素蛋白复合人工骨材料可促进骨缺损修复,具有较强的成骨能力。
BACKGROUND：To obtain a more ideal bone substitute material, nano-hydroxyapatite/silk fibroin composite material has been prepared in the preliminary experiments. OBJECTIVE：To investigate the effect of nano-hydroxyapatite/silk fibroin composite material for repair of bone defects. METHODS：Thirty healthy adult rabbits were randomly divided into two groups, and unilateral middle and distal femoral bone defect models were prepared. The experimental group was implanted with nano-hydroxyapatite/silk fibroin composite material, and the control group was implanted with pure hydroxyapatite. At 4, 8, 12 weeks after surgery, the femur was taken from each rabbit for gross, X-ray, biomechanical and histological examinations. RESULTS AND CONCLUSION： In both two groups, biomechanical strength was gradualy increased with time, but the biomechanical strength was higher in the experimental group than the control group at different time points （P 〈 0.05）. At 12 weeks after surgery, in the experimental group, the bone graft had the smooth surface and exhibited normal color with no difference from the surrounding bone, the artificial bone material was completely absorbed and degraded, and bone defects were completely healed; in the control group, the bone graft had no smooth surface on which the bone cortex was continuous and showed unclear boundary with the surrounding bone tissues, the artificial bone materials were incompletely degraded, bone defects were connected by the caluses, the artificial bone materials were closely in conjunction with the surrounding bone tissues, and bone defects were partialy repaired. These findings indicate that the nano-hydroxyapatite/silk fibroin composite artificial material can promote bone defect repair and exhibit stronger osteogenic ability.
出处
《中国组织工程研究》
CAS
北大核心
2015年第8期1190-1194,共5页
Chinese Journal of Tissue Engineering Research
基金
黑龙江省教育厅科学研究项目(11551480)~~
关键词
羟基磷灰石类
丝素蛋白
复合体
Subject headings：Hydroxyapatites
Silk
Compomers
