摘要
用抗体依赖、细胞介导的细胞毒试验(ADCC)观察了小鼠感染后不同时间血清在体外杀伤系统中对日本血吸虫童虫的作用规律。未加补体时,嗜酸粒细胞或巨噬细胞介导的童虫杀伤作用于感染后4wk出现明显作用,分别于5—7wk和6—8wk达高峰,然后下降,至11wk时降至4wk时的水平;而未加补体时无明显的中性粒细胞介导的杀伤作用。在补体参与下,这三种细胞均能介导对童虫的杀伤作用,且作用增强。证实ADCC是日本血吸虫获得性抵抗力的一个重要组分,嗜酸粒细胞、巨噬细胞介导的对童虫的细胞毒作用是不依赖补体的,而中性粒细胞介导的作用是依赖补体的。结果对选择免疫预防适宜时间和评价候选疫苗效果有参考意义。
The tests of antibody-dependent cell-mediated cytotoxicity (ADCC)were performed to observe the kinetics of in vitro killing of Schistosoma japonicum schistosomula mediated by eosinophils, macrophages or neutrophils with infected mouse sera of different duration. The results showed that when complement was not involved, the killing rates of schistosomula mediated by eosinophils or macrophages began to increase significantly at 4 wk post-infection, reached a plateau in 5-7wk and 6-8wk respectively, and then declined to the levels of that at 4wk till llwk. In case of neutrophils, there was no significant cytotoxicity detected. When complement was involved, all the three effector cells could mediate cytotoxicity to schistosomula, with the killing rates higher than ihose without complement at the correspondent time intervals. These indicate that ADCC appears to be an important ingredient in the acquired resistance to S. japonicum, and demonstrate that eosinophil- and macrophage-mediated cytotoxicity to schistosomula is complement-independent, whereas neutrophil-mediated cytotoxicity to schistosomula is complement-dependent. This method may be of some value for chosing optimal time for vaccination and estimating the effectiveness of a candidate vaccine.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1991年第3期182-185,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
国家科委863高技术计划资助项目
关键词
日本血吸虫
细胞毒试验
感染
Schistosoma japonicum, ADCC, infected mouse sera, effector cells
