摘要
目的研究东方田鼠血清蛋白组分体外杀伤日本血吸虫童虫的作用。方法采用逐级分离技术，包括硫酸铵盐析法、Sephacryl S-300分子筛层析法以及电泳洗脱法，将东方田鼠血清蛋白分离为球蛋白和白蛋白两大类，再按分子量大小依次将球蛋白分离为4个大的组分和12个小的组分，最后有重点地进行细分。然后，将分离的蛋白组分与日本血吸虫童虫共同培育48h，观察对童虫的杀伤效应。结果东方田鼠球蛋白所致的童虫死亡率为59．2％，加补体后的死亡率为68．4％，明显高于BALB／c鼠球蛋白（分别为40．8％和45．2％）；东方田鼠球蛋白中的2、3组蛋白的杀伤效果同其总球蛋白，其中的3．2、3．3、3．4组分杀伤效果分别达到45．1％、57．6％和67．2％。这3组蛋白的分子量主带为375、205kDa和100～135kDa，后者的杀伤效果接近于总球蛋白，补体有增强作用；BALB／c鼠的4组球蛋白均无明显杀伤作用。这两种鼠的白蛋白对童虫的杀伤作用无明显差异。结论东方田鼠球蛋白具有体外抗血吸虫童虫作用，其中的100-135kDa组分可能为主要效应分子。
Objective To study the killing effects of fractional proteins from Microtus fortis （M f） serum on schistosomula of Schistosorna japonicum. Methods Mf serum proteins were separated into albumin and globulin by means of salt out of ammonium sulfamate. The globulin was then separated into 4 big and 12 small fractional proteins through Sephacryl S-300 column chromatography and electrophoresis elution. The killing effects were observed in vitro in cultivation in which the purified fractional proteins and schistosomula of S. japonicum were incubated together for 48 h. Results The mortality rate of schistosomula acted by Mf globulin was 59.2% and when added with complements was 68. 4%. The killing effects of the 2nd and 3rd big fractional proteins were the same as that of Mf globulin. Three small fractional proteins （3.2, 3.3, 3.4） showed the higher killing effects which made the mortality rate of schistosomula 45.1M, 57.6% and 67. 2G, respectively. The fractional protein of 100-135 kDa also showed the same killing effect as that of Mf globulin. Globulin from BALB/c mice sera had no significant effect on schistosomula. There was no significant difference in the mortality rate of schistosomula acted by both albumins. Conclusions Mf globulin has significant killing effects on schistosomula of S. japonicum in vitro and 100--135 kDa fractional protein may be an important effective molecule.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2008年第4期260-264,共5页
Chinese Journal of Schistosomiasis Control
基金
上海市科学技术发展基金(054909002)
关键词
东方田鼠
日本血吸虫
球蛋白
白蛋白
Microtus fortis
Schistosoma japonicum
Globulin
Albumin
