摘要
目的:探讨4’-去甲峨参内酯(0号衍生物)作用于Hela、MG-63、Hep G2、A549四种肿瘤细胞后,对Bcl-2、Bax基因表达、细胞周期及凋亡产生的影响。方法以0号衍生物作为观察组,0.1%DMSO作为空白对照组,紫杉醇作为阳性对照组,分别作用48 h后采用ELISA试剂盒检测对四种肿瘤细胞Bcl-2、Bax表达的影响;采用流式细胞仪PI单标法检测细胞周期进程及凋亡的影响。结果 ELISA试剂盒检测表明,观察组及阳性对照组与空白对照组比较Bcl-2/Bax比值均明显降低,具有显著性差异(P<0.05);流式细胞仪检测0号衍生物作用四种肿瘤细胞48h后对细胞周期进程及凋亡的影响,结果表明G0/G1期细胞百分数降低,G2/M期细胞百分数基本不变,而S期细胞百分数上升,说明0号衍生物阻滞肿瘤细胞于S期,且sub-G1细胞亚二倍体凋亡峰增加,进一步说明其诱导凋亡的作用。结论 0号衍生物通过上调Bax、下调Bcl-2,促进肿瘤细胞凋亡;阻滞肿瘤细胞于S期,sub-G1峰增加,显示其具有诱导细胞凋亡的作用。
Objective： Study of 4 ＇-demethyl-4-deoxypodophyllotoxin（ No. 0derivatives） on Hela,MG-63,Hep G2,A549 four kinds of tumor cells for Bcl-2、Bax role; explore No. 0 derivatives on four tumor cells cycle and apoptosis. Methods Taking No. 0 derivatives as the observation group,0. 1% DMSO as the blank control group,paclitaxel as positive control group. Observed the changes after 48 h,respectively,the expression of Bcl-2 、Bax was detected by ELISA kit for the tumor cells; by using flow cytometry,PI single labeled effect method to detect the cell cycle and apoptosis. Results By ELISA kit testing showed that the observation group and positive control group compared with blank control group,Bcl-2 / Bax were obviously decreased,with a significant difference（ P〈0. 05）; No. 0 derivatives action of four tumor cells after 48 h effect on cell cycle and apoptosis was detected by flow cytometry,results show that the percentage of G0 / G1 phase cells decreased,G2 / M cell percentage almost constant,while the percentage of S phase cells was increased. No. 0 derivatives arrest cells in S phase,and the sub-G1 cell apoptotic peak increased,further explains its induction of apoptosis. Conclusions No. 0 derivatives by up-regulation of Bax,down-regulation of Bcl-2 to promote the apoptosis of tumor cells; and arrest cells in S phase. Sub-G1 peak increased,show its effect of inducing cell apoptosis.
出处
《中华中医药学刊》
CAS
北大核心
2015年第4期789-791,I0002,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金项目(81073036)
四川省科技厅应用基础项目(2013JY0034)
