摘要
目的探讨妊娠晚期细菌脂多糖暴露所致宫内感染大鼠发热反应和胎盘病理的变化。方法选用妊娠第18天Sprague—Dawley大鼠，随机分为对照组和宫内感染组（每组各6只），宫内感染组大鼠腹腔注射脂多糖350μg／kg建立宫内感染模型，对照组给予等体积灭菌生理盐水。腹腔注射脂多糖或生理盐水后每隔1h测定孕鼠体温，连续监测8h。于妊娠第19天麻醉后剖腹取胎盘，采用HE染色法观察胎盘组织炎症反应，酶联免疫吸附法测定胎盘组织肿瘤坏死因子-d、白细胞介素-6和白细胞介素-1β的表达水平。组间比较采用Studentt检验。结果（1）实验前2组孕鼠体温差异无统计学意义（P〉0．05）；宫内感染组孕鼠在腹腔注射脂多糖1h后体温上升，达到（37．67±0．08）℃，与对照组[（37．13±0．08）℃]比较差异有统计学意义（t=10．178，P〈0．01）；2h后发热反应逐渐消退，体温下降至37℃以下，持续呈现低体温反应，2～6h的体温分别为（37．70±0．10）、（37．23±0．05）、（36．57±0．06）、（36．60±0．10）和（36．57±0．08）℃，与对照组比较【分别为（36．83±0．12）、（36．63±0．12）、（36．71±0．07）、（36．87±0．12）和（36．77±0．08）℃]差异均有统计学意义（t值分别为11．402、11．163、-4．025、-4．000和-4．243，P值均〈0．01）。（2）宫内感染组胎盘组织HE染色显示大量中性粒细胞浸润和血管腔扩大、充血等急性炎症表现，对照组胎盘组织未见大量炎性细胞浸润。（3）宫内感染组胎盘组织肿瘤坏死因子-d[（0．62±0．02）ng／g]、白细胞介素-6[（66．12±5．11）ng／g]和白细胞介素-113[（7．09±1．23）ng／g]表达水平均明显高于对照组[分别为（0．27±0．01）、（16．71±1．55）和（2．86±0．38）ng／g]，差异均有统计学意义（t值分别为-26．608、-18．749和-5．714，P值均〈0．01）。结论孕鼠在妊娠晚期暴露于脂多糖后，胎盘存在明显的炎症反应，但其发热反应被抑制并出现低体温反应。
Objective To explore the febrile response and placental pathological inflammation of pregnant rats exposed to intrauterine infection in late gestation. Methods Pregnant Sprague-Dawley rats at gestational day 18 were randomly divided into control group and intrauterine-infected group with six rats in each. The intrauterine-infected group was intraperitoneally injected with 350 μ g/kg lipopolysaccharide to establish a rat model of intrauterine infection, while the control group was injected with sterile saline of the same dose. Core temperature was measured every 1 h after intraperitoneal injection of lipopolysaccharide or saline for 8 h. At gestational day 19, after anesthesia, the placentas were taken and stained with HE. The expression levels of tumor necrosis factor-α, interleukin-6, and interleukin-1β in the placenta were determined by enzyme linked immunosorbent assay. Student t test was used for statistical analysis. Results （1） There was no temperature difference between the two groups before experimental treatment （P 〉 0.05）. Core temperature was increased 1 h after the lipopolysaccharide injection, reaching （37.67±0.08） ~C . The increase of temperature was significant compared with the control group [（37.13 ±0.08） ℃, t=1 0.178, P 〈 0.01 ]. Fever was lowered 2 h later and the rats became hypothermic with body temperature below 37 ℃ in the group. The body temperature in the intrauterine-infected group after 2-6 h was （37.70±0.10）, （37.23 ±0.05）, （36.57±0.06）, （36.60±0.10） and （36.57 4- 0.08） ℃ , respectively, compared with the control group [（36.83 ±0.12）, （36.63 ± 0.12）, （36.71 ± 0.07）, （36.87＋0.12）, and （36.77±0.08） ℃, respectively], the differences being all statistically significant （t=- 11.402, 11.163, - 4.025, -4.000 and - 4.243, all P 〈 0.01）. （2） HE staining revealed large amounts of neutrophils infiltration, vascular enlargement and congestion in the placenta of the intrauterine infected rats. No inflammatory cell infiltration was observed in the control placentas. （3） The expression levels of proinflammatory cytokine tumor necrosis factor- α [（0.62 4- 0.02） ng/g], interleukin-6 [（66.12 4- 5.11） ng/g], and interleukin-1 β [（7.09 ± 1.23） ng/g] in the intrauterine-infected group were higher than those in the control group [（0.27 ± 0.01）, （16.71 ± 1.55） and （2.86±0.38） ng/g, respectively]. The differences were all statistically significant （t=- - 26.608, -18.749 and - 5.714, all P 〈 0.01）. Conclusion After exposure to lipopolysaccharide in late gestation, pregnant rats show significant inflammatory response in the placenta, with suppression of febrile response and presence of hypotbermia.
出处
《中华围产医学杂志》
CAS
CSCD
2015年第3期222-226,共5页
Chinese Journal of Perinatal Medicine
基金
国家自然科学基金（81471519）
湖北省自然科学基金（2012FFB02524）
关键词
妊娠并发症
感染性
发热
低温
胎盘
细胞因子类
妊娠末期
脂多糖类
疾病模型
动物
Pregnancy complications, infectious
Fever
Hypothermia
Placenta
Cytokines
Pregnancy trimester, third
Lipopolysaccharides
Disease models, animal
