摘要
目的:研究褪黑素(MT)对细菌脂多糖(LPS)致宫内感染胎鼠脑组织TNF-α的表达变化,探讨MT对宫内感染胎鼠脑损伤的保护作用。方法:选用妊娠第19天SD大鼠随机分组:对照(S)组、宫内感染(LPS)组和MT处理组,腹腔注射LPS建立大鼠宫内感染模型,LPS组和S组分别腹腔注射LPS 500μg/kg和等容积生理盐水,MT组同时腹腔注射LPS500μg/kg+MT 10 mg/kg。分别于注药后1、6、12、24、48和72 h剖腹取胎鼠,RT-PCR技术检测胎鼠脑组织中TNF-αmR-NA的表达水平。结果:LPS组各小组较S组胎鼠脑组织中TNF-αmRNA含量均明显上升,LPS组在6h达峰值之后缓慢下降;MT组各小组TNF-αmRNA含量均低于LPS组,随时间延长MT组各小组逐渐上升,48 h达峰值,后稍下降。结论:TNF-α表达增加是LPS致宫内感染胎鼠脑损伤的机制之一;MT抑制TNF-α表达,对胎鼠脑损伤有保护作用。
Objective：To research the effect of melatonin on change of tumor necrosis factor-α（TNF-α） expression in fetal rat brain tissue suffered from intrauterine infection induced by lipopolysaccharide（LPS）,and explore the protective effect of melatonin for brain injury of fetal rats with intrauterine infection.Methods：SD rats at 19 days of pregnancy were randomly divided into control group,LPS group,and melatonin group,the rats in LPS were treated with intraperitoneal injection of LPS（500 μg/kg）,the rats in control group were treated with intraperitoneal injection of normal saline（500 μg/kg）,and the rats in melatonin group were treated with intraperitoneal injection of LPS（500 μg/kg） and normal saline（10 mg/kg）.Fetal rats were obtained by laparotomy at 1,6,12,24,48,and 72 hours after injection,RT-PCR was used to detect the expression level of TNF-α mRNA in fetal rat brain tissue.Results：The contents of TNF-α mRNA in subgroups of LPS group were statistically significantly higher than those of control group,which decreased gradually after peak at 6 hours after injection;the contents of TNF-α mRNA in subgroups of melatonin group were statistically significantly lower than those of LPS group,which increased gradually with time extension,peaked at 48 hours,then decreased slightly.Conclusion：The increase of TNF-α expression is one of the mechanisms of brain injury of fetal rats with intrauterine infection induced by LPS;melatonin inhibits TNF-α expression can plays a protective role in brain injury of fetal rats.
出处
《中国妇幼保健》
CAS
北大核心
2012年第33期5378-5381,共4页
Maternal and Child Health Care of China
基金
苏州市科技发展计划(应用基础)〔YJS0928〕
关键词
褪黑素
细菌脂多糖
宫内感染
TNF-Α
Melatonin
Lipopolysaccharide
Intrauterine infection
Tumor necrosis factor-α
