摘要
为了探讨刺五加合生元对哺乳犊牛血清总抗氧化能力（T-AOC）、免疫球蛋白G（IgG）及免疫球蛋白A（IgA）含量的影响,选取健康状况良好的犊牛18头,随机分为3组,分别为试验组Ⅰ（空白对照组）、试验组Ⅱ（添加益生素组）、试验组Ⅲ（添加刺五加合生元组）;分别于犊牛的5、14日龄及21日龄颈静脉采血,3 500 r/min离心35 min,后将置于25℃～37℃温水的杯内待血清析出,-20℃保存.分析前解冻并测定血清TAOC、IgG及IgA的含量;利用SPSS 17.0统计软件对数据进行分析.结果,犊牛14日龄时,试验组ⅢT-AOC显著高于试验组Ⅰ、试验组Ⅱ（P＜0.05）;在犊牛21日龄时,试验组ⅢTAOC比试验组Ⅰ和试验组Ⅱ分别提高26.33％和31.04％..在犊牛21日龄时,试验组Ⅲ血清中的IgG含量显著高于试验组Ⅰ和试验组Ⅱ （P＜0.05）,且分别提高了21.38％和11.24％.而血清IgA的含量,犊牛21日龄时试验组Ⅲ显著高于试验组Ⅰ和试验组Ⅱ,分别提高了22.19％和9.87％.表明在对哺乳犊牛T-AOC、IgG及IgA作用上,刺五加合生元要优于益生素.
To study the effect of Acanthopanax Synbiotics on serum protein of lactation calves.18 good health of calves were se-lected and were randomly divided into three groups that were experimental group [ （control group）experimental group II （probioticgroup） and experimental group Ⅲ （Acanthopanax Synbiotics group）.The blood of jugular vein was taken on day 5,14 and 2I respec-tively and was placed in the cups with 25 ℃-37 ℃ warm water after centrifuging at speed of 3 500 r/min and serum was extracted,and then stored under -20 ℃.The T-AOC IgG and IgA were determined after the thawing of the serum. The data were analyzed bySPSS17.0 software. At age of 14days old, the content of TAOC in experimental group Ⅲ was significantly higher（P〈0.05）than that ofexperimental group I and experimental group II;At age of 21days old, the content of TAOC in experimental group BI was significant-ly higher than that of experimental group I （26.33%）and experimental group Ⅱ （31.04%）.At age of 21days old,the content of IgG inexperimental group Ⅲ was significantly higher than that of experimental group I （21.38% ） and experimental group Ⅱ （ 11.24% ） ; thecontent of IgA in experimental group Ⅲ was significantly higher than that of experimental group I （22.19%） and experimental group Ⅱ（9.87%）.Acanthopanax Synbiotics was better than probiotics in promoting the serum TAOC ,IgG and IgA of lactation calves.
出处
《中国兽医杂志》
CAS
北大核心
2015年第2期48-50,共3页
Chinese Journal of Veterinary Medicine
基金
吉林省科技发展计划项目(2014020313NY)
关键词
刺五加合生元
哺乳犊牛
Acanthopanax Synbiotics
lactation calves
serum TAOC
serum IgG and IgA
