摘要
为探讨肺癌患者体内肿瘤坏死因子 (TNF)与自然杀伤 (NK)细胞活性水平及影响二者活性的临床因素。采用外周血诱生TNF测定法检测TNF活性 ;应用荧光检测法分析细胞毒性效应DNA以观察NK细胞活性。结果发现肺癌患者NK细胞活性显著低于正常人和非癌胸病病人 ,并随病期进展、肿瘤增大及淋巴结转移而加重 ,不受病理学类型影响。TNF活性水平显著高于非癌胸病人与正常人 ,而与病期、肿瘤大小、淋巴结转移与否及病理学类型无关。结果认为肺癌病人体内细胞免疫功能处于严重失衡状态 ,表现为NK细胞活性下降 ,全血诱生TNF活性呈高水平状态。检测二者活性可为肺癌病人的综合治疗提供理论依据。
To study the changes of activities of tumor necrosis factor(TNF),natural killer cell (NKC) in patients with lung cancer and clarify the influence of clinical factors on the activities,we observed the level of TNF from peripheral blood cells by detective method of whole blood cell-derived TNF,and NKC activity was also observed with DNA-fluorometric assay for cytotoxic effect in thirty-five patients with lung cancer.The results showed that the NK activity in lung cancer patient was seriously inhibited and got worse with disease phase progress,tumor size and the extent of lymph node metastasis,but it was not associated with the pathological type.The extent and time of reduction of NK activity were related to the quality of primary lesion.As for the changes of TNF activity,PBMC (peripheral blood mononuclear cells) were activated under the presence of tumor to secrete a high level of TNF,which was not related to the stage of disease,the tumor diameter,the extent of lymph node metastasis and type of pathology.These results indicate that the cellular immune function in lung cancer patient is seriously impaired,and the function of immunoregulation is in a complicated chaotic status.We consider that the determination of the activities of TNF and NKC may provide a theoretical evidence for comprehensive treatment of patients with lung cancer.
出处
《中国医师杂志》
CAS
2000年第12期718-720,共3页
Journal of Chinese Physician
