摘要
目的:研究松潘乌头总碱的镇痛作用,并探讨其作用机制。方法:采用小鼠热板法和醋酸扭体法判断松潘乌头总碱的镇痛作用效果;通过多种工具药实验及用ELISA检测试剂盒方法测定单胺类递质含量。结果:松潘乌头总碱3mg、6mg、12mg/kg能明显提高小鼠痛阈、减少扭体次数;松潘乌头总碱12mg/kg与氟哌啶醇有协同作用,纳洛酮不能对抗其的镇痛作用,利血平能部分减弱其镇痛作用;大鼠口服松潘乌头总碱9mg/kg在外周可显著降低单胺类递质含量,在中枢能显著升高5-HT、DA含量,降低NE含量。结论:松潘乌头总碱有显著镇痛作用,该作用与调节脑内和外周血液中单胺类递质含量有关。
Objective： The paper aims to observe the analgesic of total alkaloids of Aconitum sungpanense, and preliminary studies mechanism of action. Methods： The two kinds of analgesic experiments of hot plate tests and writhing tests were adopted in mice. Using tool drugs to meas- ure analgesic effect of total alkaloids of Aconitum sungpanense and detect the cc ent of monoamine neurotransmitter by ELISA detection kit. Results： The total alkaloids of Aconitum sungpanense 3mg、6mg、 12mg/kg significantly increased the pain threshold, reduce the writhing times. The total alkaloids of Aconitum sungpanense 9mg/kg work on coordination with haloperidol, the analgesic effect cant be antagonized by naloxone, it could weaken by reserpine. Rats oral The total alkaloids of Aconitum sungpanense 9mg/kg also can increase 5-hydroxy tryptamine, dopamine of brainsteem, decrease norepinephrine acid which is the metabolic product of 5-hydroxy tryptamine significantly. Con- dusion： The total alkaloids of Aconitum sungpanense has a prominent abirritation effect. It adjusts pain through regulating the content of monoamine neurotransmitter of pain model rats induced by formaldehyde.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第6期62-65,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
兰州市科技局项目兰州市2011年创新人才团队培育计划(2011-1-140)
关键词
松潘乌头
镇痛作用
单胺类神经递质
Aconitum sungpanense Hand.-Mazz. （松潘乌头）
abirritation
mechanism action
monoamine neurotransmitter
