摘要
目的建立血浆丙泊酚浓度的高效液相色谱(HPLC)测定方法,探讨肝纤维化食蟹猴丙泊酚血浆浓度变化。方法选择肝纤维化S0、S1、S2、S3、S4期的雄性食蟹猴各12只,持续输注丙泊酚8mg·kg-1·h-1 30min。于输注1、10、20、30min和苏醒时采血,采用Agilent 1100型HPLC仪测定血浆丙泊酚浓度。血浆样品经6%高氯酸沉淀蛋白处理;色谱柱为Hypersil C18,流动相为甲醇-水(68∶32);流速1ml/min;紫外检测波长为258nm;柱温为室温;内标为麝香草酚,进样量:20μl。结果丙泊酚与血浆中其他成分分离良好,在0.1-10μg/ml范围内线性关系良好,回归方程为Ai/As=0.23C-0.045(r=0.995,n=5)。方法回收率为98.69%-100.10%,提取回收率为86.86%-89.48%。日内、日间精密度均小于5%。S4期肝纤维化组各时间点的血浆丙泊酚浓度均高于S0-S3期(P<0.05)。结论 HPLC法检测血浆丙泊酚浓度,灵敏度高,结果准确。丙泊酚代谢随食蟹猴肝纤维化程度加重而减慢。
Objective To establish a method of detecting plasma propofol concentration with high performance liquid chromatography（HPLC）, and investigate the changes of plasma propofol concentration in crab-eating monkeys with hepatic fibrosis. Methods Continuous intravenous infusion of propofol 8 mg · kg-1 · h^-1 for 30 minutes was performed in 60 crab-eating monkeys, which were onfirmed pathologically to be hepatic fibrosis in the stages of SO, S1, S2, S3 and S4 （12 monkeys in each stage）. Plasma concentration of propofol was measured at 10,20,30 min and awakening time with HPLC. The protein in blood samples was precipitated with 6% perchloric acid. Agilent 1100 HPLC instrument was used. The samples were separated on Hypersil C18 column with mobile phase consisted of methanol-water（68 ： 32） at a flow rate of 1 rnl/mitx The detection wave length was 258 nm and the column temperature was room temperature. The internal standard was thymol, and injection volume was 20μl. Results Propofol and other components in the samples were separated well with an exellent linear relation over the range of 0. 1-10μg/ml. The regression equation was Ai/As=0.23C-0. 045（r= 0. 995,n= 5）. The recovery rate for the method was 98. 69 %-100.10% and that for the extraction was 86.86%-89.48%. The precision of inter-day and intra-day was less than 5%. Plasma concentration was significantly higher in the monkeys with hepatic fibrosis in S4 stage than that in the the monkeys with hepatic fibrosis in stages of SO, S1, S2 and S3 （P〈0.05）. Conclusions Detecting plasma propofol concentration with HPLC has the advantages of high sensitivity and accuracy. The metabolism of propofol decreases as the severiey of hepatic fibrosis increases in the models of crab-eating monkey.
出处
《江苏医药》
CAS
2015年第3期249-251,共3页
Jiangsu Medical Journal
基金
国家自然科学基金(81060119)
关键词
高效液相色谱
丙泊酚
肝纤维化
High performance liquid chromatography
Propofol
Hepatic fibrosis
