摘要
为了解茶刺蛾核型多角体病毒（Iragoides asciata nucleopolyhedrovirus IrfaNPV）的增殖特性、指导茶刺蛾病毒的生产,采用室内活体增殖法,测定了IrfaNPV多角体在茶刺蛾幼虫体内的增殖动态,并设计了工艺流程,进行了大量繁殖生产试验.结果显示,IrfaNPV多角体在虫体内的增殖动态符合Logistic曲线,饲毒后9～12 d为病毒快速增殖期.按设计的生产工艺进行病毒大量繁殖生产,虫尸中病毒多角体平均可达8.3×10^8 PIB/头,茧中病毒多角体含量平均为6.4×10^8 PIB/头.该工艺可用于今后茶刺蛾病毒的大量生产.
To guide the production of lragoides fascia ta nucleopolyhedrovirus（IrfaNPV）, the propagation dynamic of IrfaNPV was investigated in laboratory. Meanwhile, the production process was developed and the mass production of the virus was conducted according to the process. The results showed the propagation dynamic of IrfaNPV in the larval body accorded with logistic curve. The average yield of IrfaNPV reached 8.3× 10^8 PIB/larva and 6.4× 10^8 PIB/cocoon, respectively. The process is expected to be applied in mass production of IrfaNPV in the future.
出处
《植物保护》
CAS
CSCD
北大核心
2015年第1期174-177,共4页
Plant Protection
基金
国家科技基础性工作专项(2013FY113200)
浙江省茶产业科技创新团队项目(2011R50024)
关键词
茶刺蛾
核型多角体病毒
增殖动态
生产工艺
Iragoides fasciata
nucleopolyhedroviruses
propagation dynamic
production process
