摘要
为揭示海洋尾丝虫在不同培养条件下的种群动力学规律,初步探讨不同温度(4,12,20,28,32℃)及不同培养液条件(虾肉浸出液、麦粒浸出液、牛肉浸膏培养液和酵母浸膏培养液)对其种群增长的影响.结果显示,不同温度下,4℃时繁殖速度慢,经历长时间(9 d左右)的停滞期进入增长期;12℃时不同培养液中的最大种群密度表现为:酵母浸膏培养液>牛肉浸膏培养液>虾肉浸出液>麦粒浸出液;20℃则表现为:牛肉浸膏培养液>酵母浸膏培养液>麦粒浸出液>虾肉浸出液.结果表明,牛肉浸膏和酵母浸膏培养液适用于短期种群的快速扩大培养,虾肉及麦粒浸出液适用于实验室保种.20℃是海洋尾丝虫生长繁殖的最适温度,而12℃的虫体种群密度稳定期维持较久.研究结果提供了实验室培养和保存海洋尾丝虫的方法,并为海水养殖动物的海洋尾丝虫病研究提供了实验生态学数据.
In order to reveal the population dynamics of Uronema marinum in different condi- tions, this paper discussed the population growth in different temperatures （4, 12, 20, 28, 32 ℃ ） and four kinds of culture mediums （ lixivium of shrimp flesh, lixivium of wheat, beef extract and yeast culture solution）. Results show that, at 4 ℃ the proliferation rate was slow, after a long stagnation period （9 d） into the growth period. The largest population density at 12 ℃ showed： yeast extract 〉 beef extract 〉 shrimp flesh 〉 wheat. The largest population density at 20 ℃ showed ： beef extract 〉 yeast extract 〉 wheat 〉 shrimp flesh . It turns out that, beef extract and yeast extract applied to rapidly expanding growth of short-term population, the lixivium of shrimp flesh and wheat would be applied to laboratory breeds conservation. 20 ℃ was the most adaptive temperature for the growth and reproduce of the U. marinum while the population could maintain population density longer at 12 ℃. These findings provided a laboratory culture and preservation method of U. marinum, and providing experimental ecological data of U. marinum in marine aqua- culture also.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第1期87-92,共6页
Journal of Fujian Normal University：Natural Science Edition
基金
国家青年自然科学基金资助项目(31201698)
福建省自然科学基金资助项目(2013J01120)
关键词
海洋尾丝虫
种群密度
种群增长
培养条件
Uronema marinum
population density
population growth
culture conditions
