摘要
目的:通过研究芫花甘草不同配伍比例的HPLC指纹图谱,探讨其化学成分的相互作用及变化趋势。方法:采用HPLC对芫花甘草不同配伍比例的指纹图谱进行比较,并用HPLC-ESI-MS对共有峰进行初步归属。结果:芫花甘草不同比例配伍对其中化学成分的溶出具有一定的规律性。随甘草比例升高,芹糖甘草苷、芹菜素5-O-β-D葡萄糖苷、芹糖异甘草苷、羟基芫花素、甘草酸的溶出降低;芹菜素-7-O-β-D-葡萄糖醛酸苷,在1∶1配伍比例中溶出最多。结论:芫花甘草配伍后成分的溶出降低,从而可能降低其药理作用,从化学成分角度研究评价中药配伍禁忌提供思路和方法。
Objective：To investigate the interaction and changing trend of chemical composition in eighteen incompatible medicaments,Genkwa Flos and Glycyrrhizae Radix,with different compatibility using HPLC fingerprints.Method：The fingerprints of Genkwa Flos and Glycyrhizae Radix with different compatibility proportion were analyzed by HPLC.Characteristic peaks were identified preliminarily based on the MS spectra and literature data.Result：The dissolution of liquiritin apioside,apigenin-5-O-β-D-glucoside,isoliquiritin apioside,hydroxy-genkwanin and glycyrrhizic acid were reduced with increase the proportion of Glycyrhizae Radix.The dissolution of apigenin-7-O-β-D-glucuronide was reached the maximum value at 1 ∶ 1 compatibility proportion.Conclusion：The dissolution of Genkwa Flos and Glycyrrhizae Radix reduced,thus may reduce its pharmacological action,provide the mentality and the method of evaluation of traditional Chinese medicine incompatibility from chemical angle.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第2期80-82,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
十八反
芫花
甘草
高效液相色谱
Genkwa Flos
Glycyrrhizae Radix
eighteen incompatible medicaments
HPLC
