摘要
目的:探讨上皮间质转化(EMT)是否参与姜黄素抑制乳腺癌细胞侵袭转移过程。方法:利用不同浓度姜黄素干预脂多糖(LPS)诱导的乳腺癌细胞MCF-7,MTT法检测细胞增殖能力,普通光学显微镜及透射电子显微镜观察细胞形态变化,RT-PCR和Western blot方法分别检测vimentin,E-cadherin表达变化。结果:姜黄素可抑制LPS诱导的乳腺癌细胞MCF-7 EMT的发生,降低LPS诱导的EMT标志物vimentin蛋白的表达,增强E-cadherin的表达,最终导致乳腺癌细胞运动及侵袭能力的降低。结论:姜黄素可以抑制肿瘤细胞侵袭能力,提示EMT程序参与其中,具体分子机制仍需进一步研究。
Objective： To investigate whether EMT is implicated in the anti-invasion and metastasis effects of curcumin on MCF-7 breast cancer ceil line. Methods： MTT was used to de- tect the anti-proliferative effect of curcumin on MCF-7 cell. The morphological changes were de- termined using optical and transmission electron microscopy, respectively. The protein level for vi- mentin, E-cadherin, were analyzed using RT-PCR and Western blotting. Results： Lipopolysaccha- ride（LPS） was used to trigger EMT in MCF-7 breast cancer cell lines and showed that curcumin in- hibited LPS-induced morphological changes, decreased the expression of LPS-induced markers of EMT such as vimentin, and increased the expression of E-cadherin, resulting in the inhibition of in vitro cell motility and invasiveness. Conclusion： These data provide a new perspective of the an- ti-invasion mechanism of curcumin, indicating that the effect is in part due to its ability to attack the EMT process.
出处
《中国现代普通外科进展》
CAS
2014年第11期851-856,共6页
Chinese Journal of Current Advances in General Surgery
