摘要
目的观测大鼠-骨骼肌损伤后不同时间点的病理学变化,为损伤时间推断的研究和应用提供依据和参考。方法 SD大鼠40只随机分为实验组(35只)和对照组(5只),实验组用500g重的打击器打击大鼠右下肢,建立大鼠骨骼肌机械性损伤动物模型,并随机均分为伤后6h、12h、1d、3d、7d、10d、14d组。应用HE染色、Mallory染色和图像分析法观测大鼠骨骼肌损伤的病理学改变。结果伤后6h,损伤区骨骼肌变性、间质大量出血和炎细胞浸润;12h^1d,骨骼肌坏死,伴大量炎细胞浸润,以PMNs和MNCs为主,数量分别达到高峰;3d起,坏死骨骼肌被逐渐清除,PMNs和MNCs数量逐渐减少,FBCs和新生骨骼肌开始出现,少量胶原纤维形成;7d,FBCs和新生骨骼肌数量继续增多,伴新生血管生成和胶原纤维沉积;10d^14d,FBCs数量和胶原纤维沉积分别达到高峰。结论大鼠骨骼肌损伤区PMNs、MNCs和FBCs数量,以及胶原纤维沉积随损伤经过时间的规律性变化,可为损伤经过时间推断研究提供参考。
Objective To observe pathological changes in different post-traumatic intervals after rats skeletal muscle injury and to provide reference for investigating skeletal muscle wound age determination in future. Methods A total of 40 Sprague-Dawley male rats were divided into control and injured groups （6h, 12h, ld, 3d, 7d, 10d and 14d after injury）. We established a standardized animal model of skeletal muscle contusion in rats, which was controllable and reproducible well using a self-designed mechanical weight-drop （500g） device. A single impact was delivered to the site of the fight posterior limb. Pathological changes after rats skeletal muscle injury were investigated by hematoxylin-eosin （HE） staining, Mallory staining and image analysis. Results Degeneration, hemorrhage, and inflammatory infiltration were present in contused skeletal muscle at 6h post-injury; neerotized skeletal musele fibers were infiltrated by a large number of inflammatory eells, especially PMNs and MNCs. The number of PMNs and MNCs infiltrated into injured sites reached respectively a peak at 12h ld post-injury; from 3d post-injury onward, neerotized skeletal muscle fibers were phagoeytosed gradually. And the number of PMNs and MNCs was decreased gradually. FBCs, collagen fibers and regenerating myofibers were present in injured sites; the number of FBCs and regenerating myofibers augmented remarkably at 7d post-injury. Simultaneously, angiogenesis and collagen fibers were also observed; at 10 - 14d post-injury, the number of FBCs and collagen fibers deposition reached respectively a peak. Conclusion The number of PMNs, MNCs, FBCs, and the percentage of collagen fibers deposition in skeletal muscle injured zones was respectively time-dependent changes, which may provide reference for wound age determination in forensic medicine.
出处
《中国法医学杂志》
CSCD
2014年第5期431-433,436,I0001,共5页
Chinese Journal of Forensic Medicine
基金
国家自然科学基金青年科学基金项目(81202383)
教育部高等学校博士学科点专项科研基金项目(20122104110025)
中国政法大学青年教师学术创新团队资助项目
关键词
法医病理学
损伤时间推断
多形核白细胞
单个核细胞
成纤维样细胞
胶原纤维沉积
forensic pathology
wound age determination
polymorphonuclear leukocytes
mononuclear cells
fibroblastic cells
collagen fibers deposition
