摘要
目的探讨云南地区彝族、哈尼族人群NINJ2基因多态性与脑卒中的相关性。方法选择云南地区彝族脑卒中患者9例、健康对照者37例及哈尼族脑卒中患者7例、健康对照者89例,采用聚合酶链式反应—限制性片段长度多态性(PCR-RFLP)技术检测NINJ2基因两个SNP位点(rsl2425791、rsll833579)的基因型,比较各组间的基因型及等位基因分布频率是否存在差异。结果彝族人群中两组比较,基因型及等位基因频率差异无统计学意义。哈尼族人群中两组比较,rsll833579等位基因(A,G)频率差异有统计学意义(P<0.05),基因型差异无统计学意义,rsl2425791基因型及等位基因频率差异均无统计学意义。结论 NINJ2基因的rsll833579位点的等位基因(A,G)频率与哈尼族人群脑卒中存在相关性,其基因型以及rsl2425791位点的等位基因频率及基因型与哈尼人群脑卒中无相关性。NINJ2基因两个SNP位点(rsl2425791、rsll833579)与彝族人群的脑卒中无相关性。
Objective To explore the association between the NINJ2 gene polymorphism and stroke in the Yi and Hani nationality of Yunnan region. Methods We selected 9 cases of stroke and 37 healthy controls from the Yi nationality as well as 7 cases of stroke and 89 healthy controls from the Hani nationality in Yunnan Province. Polymerase chain reaction - restriction fragment length polymorphism （PCR- RFLP） method was used to detect the genotypes of the 2 single nueleotide polymorphism （SPN） sites （rsl2425791 and rsll833579） in NINJ2 gene. The differences of genotypes and alleles frequencies of the 2 SNP sites between each 2 different groups were analyzed and compared. Results There was no statistically significant deviation about the genotypes and allele frequencies of the 2 SPNs between healthy controls and stroke patients in the Yi nationality. There were statistically significant differences in the allele frequencies （A and G） of rsll833579 site between healthy controls and stroke patients in the Hani nationality （P〈 0.05）, but no statistically significant difference was found in the genotypes of rsll833579 site as well as the genotypes and allele frequencies of rsl2425791 site between the above mentioned two groups. Conclusions The allele frequencies （A, G） of rsll833579 site in NINJ2 gene are corrdated with stroke in the Hani nationality of Yunnan Province, whereas the genotypes of rsll833579 site as well as the allele frequencies and genotypes of rsl2425791 site are not associ- ated with stroke in the Yi nationality of Yunnan Province. There is no correlation between the SPN sites （rsl2425791 and rsl1833579） in NINJ2 gene and stoke in the Yi nationality of Yunnan Province.
出处
《实用预防医学》
CAS
2015年第1期34-37,共4页
Practical Preventive Medicine
基金
云南省卫生科技内设研究机构基金资助(基金编号:2011WS0014)
