摘要
为了解贵州魔芋软腐病的致病病原,对取自贵州台江县的魔芋软腐病病原菌进行了分离、回接和鉴定。结果表明:在分离到的16个菌株中发现1株病原菌能引起魔芋软腐病,通过16SrDNA序列分析发现该病原菌为菊欧文氏杆菌(Erwinia chrysanthemi);通过化学转化法,成功获得了病原菌绿色荧光蛋白标记的工程菌株。
The pathogenic bacteria strains of Amorphophallus rivieri soft rot from Taijiang County,Guzhou were isolated,inoculated and identified to study the pathogen of Amorphophallus rivieri soft rot in Guizhou.The results showed that one pathogenic bacteria strain to cause Amorphophallus rivieri soft rot is isolated from 16 strains,and the strain is identified as Erwinia chrysanthemi by 16 SrDNA sequence analysis.The engineering strain with GFP marker is successfully obtained by the chemical conversion method.
出处
《贵州农业科学》
CAS
北大核心
2014年第12期118-121,共4页
Guizhou Agricultural Sciences
基金
贵州省农业委员会项目"魔芋栽培技术研究"[黔农发(2013)167]
贵州省农业科学院科技成果培育与人才培养项目"魔芋软腐病入侵特征及防治药剂筛选与示范"[黔农科院CR合字(2014)38]
贵州省科技厅农业科技攻关项目"魔芋防病高效栽培技术研究与示范"[黔科合NY(2012)3044]
关键词
魔芋
软腐病
鉴定
GFP标记
Amorphophallus rivieri
soft rot
identification
GFP marker
