摘要
本文提出了一种基于超声辅助离子液体分散液液微萃取紫外分光光度法测定药物及人血清中氯诺昔康含量的方法。疏水性离子液体1-辛基-3-甲基咪唑六氟磷酸盐（[ C8 mimPF6]）被用作萃取剂,在超声的帮助下,不使用分散剂离子液体即可萃取氯诺昔康,萃取率在85．0%以上。对实验条件如萃取剂的种类和用量、萃取时间、萃取温度、溶液pH、离心时间等进行了优化。在优化条件下,工作曲线的线性范围为0．2~15．0μg ·mL^-1,检测限为0．032μg·mL^-1,加标回收率分别为94．5%~99．0%（药物）和92．2%~98．7%（人血清）。用本方法对氯诺昔康药片和加标后的人血清样品进行了测定,结果令人满意。
In this paper,a simple and efficientmethodology,ultrasound-assisted ionic liquid dispersive liquid-liquid microextraction （ UA IL-DLLME） coupled with ultraviolet spectrophotometry has been developed and applied to the separation/determination of lor-noxicam in tablet and human serum samples. The hydrophobic IL[ C8 MIM] [ PF6 ] was used as extraction solvent and lornoxicam can be easily migrated into IL phase with help of ultrasonication without any dispersive solventand the extraction efficiency was over 85. 0%. Several important parameters affecting the extraction efficiency were studied and optimized,including the type and volume of extraction solvent,sample pH,ultrasonication time and temperature and centrifugation time. Under the optimized conditions,the calibration curve was linear over the range of 0. 2~15. 0 μg·mL^-1 and the limit of detection（LOD）was 0. 032μg·mL^-1. The pro-posed method was successfully applied tothe spiked samplesof lornoxicam tablet and human serum samples and good recoveries in the range of 94. 5% ~99. 0%（ tablet） and 92. 2% ~98. 7%（ human serum） were obtained.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2014年第12期1859-1863,共5页
Chemical Research and Application
基金
扬州工业职业技术学院校级课题(2013xkj15)资助
关键词
氯诺昔康
离子液体
液液微萃取
lornoxicam
ionic iquid
liquid-liquid microextraction
