摘要
目的:探讨沉默RABGEF1基因对人卵巢癌细胞(SK-OV-3细胞)增殖的影响。方法:通过小干扰RNA技术沉默SK-OV-3细胞的RABGEF1基因表达,用实时荧光定量RT-PCR法鉴定细胞内RABGEF1基因的表达水平,培养RABGEF1基因被沉默的SK-OV-3细胞,计数不同时间点的细胞数,分析RABGEF1基因对人卵巢癌细胞增殖的影响。结果:实时荧光定量RT-PCR法检测显示所构建的4个siRNA表达载体对RABGEF1基因表达的沉默率分别为52.5%、58.1%、76.2%和87.7%。将RABGEF1基因表达沉默率为87.7%的SK-OV-3细胞继续培养,计数显示细胞数目较未沉默基因减少(P<0.05)。结论:应用RNAi技术沉默RABGEF1基因可抑制SK-OV-3细胞的增殖,提示RABGEF1基因可能参与卵巢癌的发生发展。
Objective： To explore effect of silencing the RABGEF1 gene expression on cell proliferation of ovarian cancer cell（ SK-OV-3）. Methods： Expression of RABGEF1 gene was silenced by small interfering RNA in SK-OV-3 cells. Expression level of RABGEF1 gene was determined by real-time quantitative RT-PCR in cultured SK-OV-3 cells. The number of SK-OV-3 cells transfected with the small interfering RNA expression vectors was counted at different times. Effect of the RABGEF1 gene expression on cell proliferation of SK-OV-3 cells was estimated by cellnumber. Results ： The silencing rates of the RABGEF1 gene were 52. 5%,58. 1%,76. 2% and87. 7%,respectively,by real-time quantitative RT-PCR for four small interfering RNA expression vectors. The number of SK-OV-3 cells transfected with the small interfering RNA expression vectors with 87. 7% silencing rate reduced（ P 0. 05）. Conclusion： Silencing the RABGEF1 gene by RNAi can suppress cell proliferation of SK-OV-3 cells,it suggests that RABGEF1 gene may have a role in occurrence and progression of ovarian cancer.
出处
《现代肿瘤医学》
CAS
2014年第12期2809-2811,共3页
Journal of Modern Oncology
基金
深圳市科技计划项目(编号:201202181)
