摘要
为了揭示ADP-葡萄糖焦磷酸化酶(AGPase)改变对叶片光合作用的反馈调控,以改变AGPase活力的马铃薯转化株系为材料,测定了其AGPase活力(AA)、块茎淀粉积累量(SC)、叶片光合速率(PR)、叶绿素(CC)和蔗糖含量(SUC)等;测定结果分析显示,各株系的AGPase活力(AA)、块茎淀粉积累量(SC)和叶片光合速率(PR)间存在显著差异,且均显著高于对照;株系间AGPase活力、块茎淀粉含量和叶片光合速率呈显著正相关性;每单位AGPase活力上升可贡献贮藏器官淀粉积累增加2.5%,且是影响叶片光合速率的重要因素;结果表明,AGPase不仅是下游淀粉积累的限速酶,而且可向上游反馈调控光合速率。
To reveal feedback regulation of ADP-pyrophosphrolase（AGPase）to photosynthesis, AGPase activities（AA）, starch contents（SC）in tuber, photosynthetic rates（PR）, chlorophyll contents（CC）and sucrose contents（SUC）in leafs were assayed with potato transgenic lines showing difference on ADP-pyrophosphrolase activities. The data analysis showed that AA, SC and PR in transgenic lines were significantly higher than control line. Significantly differences were found between AA, SC and PR among the lines. Correlation analysis showed that there were positive correlations between AA, SC and PR. Per unit rising of AGPase activities can contributed 2.5% increase of starch contents in sink tissue. AGPase was a factor playing a role in regulation of photosynthetic rates. The result indicated that AGPase not only regulates starch accomulation on the downstream, also feedback to modulate photosynthetic rate.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第11期125-129,共5页
Biotechnology Bulletin
基金
国家自然科学基金项目(30660079)
2012年宁夏科技支撑计划
