摘要
利用SRAP标记对来自海南省的28份旱稻种质进行了亲缘关系分析。从255对引物组合中筛选出46对扩增条带清晰、多态性高的引物组合,并选用其中的18对引物组合对28份材料进行SRAP扩增,共扩增出180个条带,其中多态性条带160条,多态性比率为87.75%,平均每对引物组合产生10个条带和8.9个多态性条带。并且,运用UPMGA方法进行的聚类分析结果表明,供试旱稻种质的遗传相似系数范围为0.687 0-0.921 2,在遗传相似系数0.687 0处分为两大类群,第I类群全部为海南山栏稻种质,第Ⅱ类群为海南地方旱稻坡压和本研究组自育旱稻品种。对海南旱稻地方品种的划分表明,山栏稻种质间亲缘关系较近,海南地方旱稻坡压可能与籼型水稻种质具有一定的遗传相似性。此外,还构建了28份海南省旱稻种质的SRAP标记分子身份证,可为山栏稻种质的遗传亲缘关系,种质鉴定及新品种选育等方面提供依据。
SRAP markers were used to analyze the genetic relationships of 28 upland rice local varieties from Hainan province. Forty-six primer combinations with abundant polymorphism were selected from 255 primer combinations, and 18 primer combinations were picked to amplify the 28 material. A total of 180 bands were scored, and 160（87.75%）out of them were polymorphic, with an average of 10 bands and 8.9 polymorphic bands for each primer combination. Cluster analysis using UPMGA method showed that the genetic similarity coefficient ranged from 0.687 0 to 0.921 2 among the test upland rice varieties. The tested materials were classified into two clusters at the similarity coefficient of 0.687 0, and cluster I included Hainan shanlan upland rice;the cluster Ⅱ involved upland rice poya and varieties developed by our team. The clustering result of Hainan upland rice varieties showed close genetic relationship among Shanlan upland rice varieties. The Hainan upland rice poya might has some genetic similarities with nonglutinous rice germplasms. In addition, we established the molecular identity card of 28 Hainan upland rice varieties with SRAP markers. All these results provided the basis for the Shanlan upland rice heredity relationship and the Shanlan upland rice germplasm resources’ appraisal and the new variety’s breeding.
出处
《生物技术通报》
CAS
CSCD
北大核心
2014年第11期97-106,共10页
Biotechnology Bulletin
基金
海南省自然科学基金项目(808114)
海南大学2009科研项目(hd09xm54)
关键词
旱稻
山栏稻
SRAP标记
遗传多样性
分子身份证
Upland rice
Shanlan upland rice
SRAP markers
Genetic diversity
Molecular identity card
