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毒素蛋白基因mazF在基因修饰系统中的应用进展 被引量：5

Application Progress of mazF Gene in Gene tic Modification System
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摘要 毒素-抗毒素系统(Toxin-antitoxin system,TA系统)存在于大部分细菌中。mazEF是大肠杆菌中一种毒素-抗毒素系统。毒素基因mazF编码的MazF毒素蛋白可以特异性地剪切自由mRNA的ACA序列,从而抑制蛋白合成、引起细胞生长停滞;近些年,许多学者利用mazF基因作为反向筛选标记对不同种微生物建立了无标记或无痕的基因修饰系统,并实现了不同菌株的基因组修饰。主要综述了大肠杆菌mazF基因作为反向筛选基因的应用原理及其在不同种类微生物的基因修饰系统中的应用进展,然后对mazF基因及其他毒素基因在基因修饰系统中的应用进行了展望。 Toxin-antitoxin system（TA）exists in most of the genetic material of bacteria.mazEF is a kind of toxin-antitoxin system in E. coli. MazF encoded bymazF is an mRNA interferase that specifically cleaves free mRNAs at ACA sequences, resulting in inhibited protein synthesis and cell growth arrest. Recently, some scholars have usedmazF as a counter-selection marker in genetic modification systems, and achieved modifications of the genome in various strains. In this article, the research advances of E. coli mazF used as counter-selection marker in bacterial genetic modification were reviewed, followed by application of other toxin gene. Finally, perspective of the possible new pathways for developing new genetic modification methods were addressed.
作者 石杨 董会娜 张大伟
机构地区 天津大学药物科学与技术学院 中国科学院天津工业生物技术研究所
出处 《生物技术通报》 CAS CSCD 北大核心 2014年第11期48-54,共7页 Biotechnology Bulletin
基金 国家自然科学基金项目(31200036 31370089) 天津市科技支撑计划重点项目(12ZCZDSY12700 11ZCZDSY08500)
关键词 毒素蛋白基因 反向筛选标记 基因修饰方法 mazF mazFToxin gene Counter-selection marker Genetic modification method
分类号 Q933 [生物学—微生物学]
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参考文献35

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同被引文献75

  • 1余秉琦,沈微,诸葛健.适用于异源DNA高效整合转化的谷氨酸棒杆菌电转化法[J].中国生物工程杂志,2005,25(2):78-81. 被引量：26
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  • 7Tadele A, Beyene D, Hussein J, et al. Immunocytochemical detection of Mycobacterium tuberculosis complex specific antigen, MPT64, improves diagnosis of tuberculous lymphadenitis and tuberculous pleuritis[ J/OL]. BMC Infect Dis, 2014, 14 : 585.
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  • 9Ren D, Kordis AA, Scnenshine DE, et al. The ToxAvapA toxin-antitrdn locus contributes to the survival of nontypeable Haemophilus influenzae during infection[J/OA]. PLoS One, 2014, 9(3) : e91523.
  • 10Singh R, Barry CE 3rd, Boshoff HI. The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specificeffects on bacterial antibiotic tolerance [ J]. J Bacteriol, 2010, 192 (5) : 1279 - 1291.

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生物技术通报
2014年 第11期

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