摘要
目的:建立酸枣仁皂苷A、B含量测定的前处理及分析方法,测定不同产地所产酸枣仁中皂苷A、B的含量。方法:采用快速溶剂萃取法(Accelerated Solvent Extraction,ASE)从酸枣仁中提取皂苷A、B,优化快速溶剂萃取法的提取条件以确定最佳条件,对蒸发光散射检测器(Evaporative Light Scattering Detector,ELSD)检测参数优化后,采用高效液相色谱(High Performance Liquid Chromatography,HPLC)测定七种不同产地酸枣仁中的皂苷A、B含量。结果:快速溶剂萃取最佳提取条件:两步提取,第一步提取的最佳条件是乙醇浓度60%、提取频率1次/min、提取压力100bar、提取温度100℃,第二步提取的最佳条件是乙醇浓度60%、提取频率5次/min、提取压力120bar、提取温度100℃,各地酸枣仁皂苷总含量大小依次为:河北白平>河北赞皇>山东>河北富平>辽宁>老挝>缅甸。结论:快速溶剂萃取法和HPLC-ELSD法测定酸枣仁皂苷回收率高、准确性好,产于河北、山东的酸枣仁中皂苷含量远高于其他地方的酸枣仁,可以作为开发酸枣仁保健品的优质原料使用。
Objective：To develop the method of pretreatment and analysis for content determination of jujuboside A and B in Zizyphi Spinosi Semen（ZSS）,to study the contents of jujuboside A and B in ZSS from different regions.Methods： Accelerated solvent extraction（ASE）was used as method of extracting jujuboside A and B from ZSS and the conditions of ASE were optimized after parameters of ELSD were optimized and HPLC analysis method was tested, contents of jujuboside A and B in ZSS from seven different origins were determined.Results：The optimal ASE conditions=two-step extraction,optimum conditions of the first step were 60% ethanol,1 times/min（extraction frequency）,]00 bar（extraction pressure） ,100℃（extraction temperature）, optimum conditions of the second step were 60% ethanol,5 times/min, ]20 bar,100℃,the order of jujuboside contents in ZSS from different origins as follows.HeBei Baiping 〉 HeBei Zanhuang 〉 ShanDong 〉 HeBei Fuping 〉 LiaoNing 〉 Laos 〉 Myanmar.Conclusion. HPLC-ELSD combined with ASE method is very suitable for determination of jujubosides A and B in ZSS with high recovery rate and good accuracy;ZSS produced in HeBei and ShanDong in contents of jujubosides A and B is far higher than other ZSS,and these two ZSS can be used as high quality raw materials for researching and developing health care products.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第23期265-270,共6页
Science and Technology of Food Industry
基金
北京市自然科学基金(14L00184)
北京市2013年本科生科学研究计划项目
北京市重点建设学科资助项目(PXM2009-014207-078172)
北京农学院2012年度科研质量提高经费招标项目(QKZ 2012003)
食品质量与安全北京实验室2014年度开放课题
2014年北京农学院学位与研究生教育改革与发展项目
