摘要
目的：研究不同来源副溶血性弧菌的生物膜形成及粘附情况,并对多因素影响副溶血性弧菌成膜进行探究。方法：采用改良试管法和微孔板法对不同影响因子（p H 6.5~9.5;Na Cl 2%~4%）下21株副溶血性弧菌的生物膜粘附程度和生物膜量进行测定,然后运用改良微孔板法对成膜效果较好的菌株以及标准菌株Vp BJ1.1616的成膜特性进行初步研究。结果：21株菌在实验条件下成膜能力与菌株来源和细菌致病性并没有表现出明显相关性,且Vp F29成膜效果最好;在p H6.5~9.5,Na Cl 2%~4%条件下,不同菌株平均OD570nm值变异系数（CV=标准偏差/平均值）分别从0.357变化到0.447,从0.383变化到0.472;在胰蛋白胨大豆肉汤（TSB）浓度为2.4%~9.6%时,Vp F29在32~37℃条件以及Vp BJ1.1616在37℃条件下都能形成大量生物膜。结论：不同环境条件下的成膜情况说明,副溶血弧菌成膜能力的强弱具有菌株多样性以及特性规律,为研究生物膜引起的食品安全问题提供理论依据。
Objective：The aim of this study was to investigate the biofilm-forming ability and various adhesion influence factors of different Vibrio parahaemolyticus strains.Methods.. Using modified tube test and microtiter-plate test methods,the biofilm adhesion and OD57o value of the 21 strains were made under various factors,then the best biofilm formation strain and a typical strain VpBJt.t616 were chose for further characteristic research using modified microtiter-plate test.Results：No obvious correlation were found between biofilm formation and source or pathogenicity of strains, and the strain VpF29 from food was the best among the 21 strains.The coefficient of variation（CV = standard deviation/mean ＊ 100）of OD570nm among the tested strains at pH （6.5-9.5）was from 0.357 to 0.447,and at NaCI（2% ~4% ）was from 0.383 to 0.472.1t was also found that both VpF29 and VpBJ1.1616 biofilm strongly adhered to the Polyvinyl chloride （PVC） surface at the following conditions ： 32 ~ 37℃, 37 ℃ separately, the content of TSB was 2.4% to 9.6%.Conclusion：These data showed that Vibrio parahaemolyticus biofilm formation had strain variability and specialty,which provided a basis on the study of the relation between food safety and biofilm.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第23期163-167,共5页
Science and Technology of Food Industry
基金
上海市科委工程中心建设项目(11DZ2280300)
上海海洋大学研究生科研基金资助
关键词
副溶血性弧菌
生物膜形成
影响因子
菌株多样性
Vibrio parahaemolyticus
biofilm formation
influence factors
strain variability
