摘要
目的:通过检测IL-24和HLA-G蛋白在孕妇血清及胎盘中的表达,探讨IL-24与HLA-G蛋白在子痫前期中的表达意义。方法:采用ELISA法和免疫组化SP法,对30例正常孕妇和35例子痫前期患者的血清和胎盘组织中IL-24、HLA-G蛋白表达水平进行检测,并分析两者的相关性。结果:1与对照组相比,子痫前期组血清中IL-24表达显著升高(P<0.01),HLA-G表达显著降低(P<0.01)。2血清中IL-24、HLA-G的表达呈负相关(r=-0.976,P<0.05)。3子痫前期组胎盘组织中IL-24的阳性表达率高于对照组(P<0.05),HLA-G蛋白的阳性表达率低于对照组(P<0.05)。结论:IL-24、HLA-G可能参与了子痫前期的发生和病理生理过程。
Objective： To explore the expressions and significance of IL - 24 and HLA - G in preeclampsia by detecting the ex- pressions of IL - 24 and HLA - G in maternal serum and placental tissue of patients with preeclampsia. Methods： ELISA and immunohistochemical SP method were used to detect the expression levels of IL - 24 and HLA - G in maternal serum and placenta of 30 normal pregnant women （control group） and 35 women with preeclampsia （preeclampsia group）, the correlation was analyzed. Results： Compared with con- trol group, the expression level of IL - 24 in serum of preeclampsia group increased significantly （ P 〈 0. 01 ）, the expression level of HLA - G decreased significantly （P 〈0.01 ） . There was a negative correlation between IL- 24 expression and HLA -G expression in serum （r = - 0. 976, P 〈 0. 05 ） . The positive expression rate of IL - 24 in placental tissue of preeclampsia group was statistically significantly higher than that in control group （P 〈 0. 05 ）, while the positive expression rate of HLA - G in placental tissue of preeclampsia group was statistically significantly lower than that in control group （P 〈 0. 05 ） . Conclusion ： IL - 24 and HLA - G may be involved in occurrence and patho- genesis of preeclampsia.
出处
《中国妇幼保健》
CAS
北大核心
2014年第33期5478-5480,共3页
Maternal and Child Health Care of China
基金
广西自然科学基金〔2010GXNSFCO13015〕
