摘要
目的:探讨青海高原地区子痫前期患者人类白细胞相关抗原G(HLA-G)mRNA、蛋白表达情况及临床意义。方法:收集2011年9月至2011年12月在青海大学附属医院产科分娩的30例子痫前期患者和30例正常妊娠妇女的胎盘组织和血清样品,采用实时荧光定量PCR及West-ernblot分析检测HLA-GmRNA和蛋白在正常妊娠与子痫前期患者胎盘中的表达差异,并应用双抗体夹心酶联免疫吸附法(ELISA)检测血清中可溶性HLA-G的表达。结果:①青海高原地区子痫前期患者胎盘组织中HLA-GmRNA、蛋白表达水平明显低于正常妊娠组,差异有统计学意义(P<0.05)。②子痫前期患者血清中可溶型HLA-G(sHLA-G)浓度明显低于正常妊娠组,差异有统计学意义(P<0.05)。结论:HLA-G基因转录的下调及其蛋白质表达下降,在青海高原地区子痫前期的发生发展过程中可能发挥重要作用。
Objective：To investigate the expression and the clinical significance of HLA-G at the mRNA and the protein levels in pre-eclampsia patients at the plateau area of Qinghai. Methods：The placental tissue and serum sample of 30 normal pregnant women and 30 patients with pre-eclampsias were collected. Using real time polymerase chain reaction and Western blot analysis, we detected HLA-G mRNA and protein levels in placental tissues. Serum sample was tested for soluble HLA-G （sHLA-G） protein by using a validated sandwich ELISA. Results：①The expressions of HLA-G mRNA and protein in pre-eclampsia group were sig- nificantly lower than those in control group （ P 〈 0.05）.②The maternal serum concentrations of sHLA-G in pre-eclampsia group were significantly decreased compared with those in normal pregnant women. Conclu- sions： The decreasing of HLA-G mRNA and protein expressions may play an important role in the occur- rence and development of pre-eclampsia in the plateau area.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2012年第8期671-674,共4页
Journal of Practical Obstetrics and Gynecology
