摘要
目的构建Caco-2单层细胞转运5种毒素的吸收率模型并验证。方法以Caco-2细胞系为工具，以脱氧雪腐镰刀菌烯醇（DON）、黄曲霉毒素B1（AFB1）、AFB2、黄曲霉毒素G1（AFG1）和AFG2为研究对象，通过对转运介质溶液中毒素的初始浓度、温度、pH值、抑制剂存在与否等实验条件的优化，构建Caco-2细胞转运DON和4种黄曲霉毒素的生理模型并进行验证。结果DON、AFB1、AFB2、AFG1及AFG2透过Caco-2单层细胞的量和表观渗透系数（Papp）随着体系溶液中pH的升高有下降趋势。AFB，在pH7．4—7．5溶液中的Papp值最高，AFB2、AFG1、AFG2及DON在pH7．0～7．1溶液中的Papp值最高。随着体系温度的升高，DON、AFB1、AFB2、AFG3及AFG2的透膜能力均有所增加。无论体系中AFB1、AFG1及AFG2浓度低或高，均在60min时透过率达到平台期并维持稳定；而AFB，及DON从高浓度到低浓度均在120min时透过率达到平台期并维持稳定。结论所建5种毒素Caco-2单层细胞模型灵敏、稳定、可靠、有效。
Objective The Caco-2 cell model for assessing the bioavailabilities of mycotoxins was developed and validated. Methods The Caco-2 monolayer cell transferring model was developed and validated by optimizing the initial concentrations of toxins in the apical side, the temperature and pH values of the experiment system, and the presence of mycotoxin adsorbent. Results The apparent permeability coefficient（Papp） of AFB1 reached the highest when pH value was in the range between 7.4 and 7.5, while the Papps for DON, AFB2, AFG1 and AFG2were at the highest in the pH value between 7.0 and 7.1, respectively. The permeabilities of these five mycotoxins were increasing along with the temperature ascending. The transmicivities of AFB1, AFG, and AFG2 reached the highest at the incubation time of 60 minutes but 120 minutes for DON and AFB2. Conclusion The developed Caco-2 transferring model of the five mycotoxins was susceptible, stable, effective and reproducible.
出处
《卫生研究》
CAS
CSCD
北大核心
2014年第6期923-928,共6页
Journal of Hygiene Research
基金
国家自然科学基金(No.30872124)
