摘要
目的建立干酪乳杆菌细胞壁成分(LCWE)诱导的川崎病动物模型,为后续川崎病的相关研究提供实验材料。方法LCWE组小鼠经腹部及肌肉注射LCWE,每只注射0.1mL(含200mg LCWE),PBS组小鼠注射0.1mL PBS,分别于注射后第1、3、28天对小鼠进行眼眶取血,并解剖取心、肺、肝、肾、脾等脏器组织进行病理切片、HE染色,观察组织病理变化,并对小鼠血液进行血常规检验。结果 LCWE组在注射LCWE后第1、3、28天,WBC逐渐上升,在注射LCWE后第3天PLT及MPV上升,第28天基本恢复正常。病理切片显示LCWE组小鼠心脏组织可见血管壁增粗且周围有炎症细胞浸润,有些可出现斑块。结论成功建立了川崎病动物模型。
Objective To establish mice models of Kawasaki disease by Lcatobacillus casei cell wall extract（LCWE）,in order to provide experimental materials for follow-up study.Methods LCWE group was given LCWE 0.1mL per mouse（containing 200 mg LCWE）via abdominal intramuscular injection.PBS group was given 0.1mL PBS per mouse.Orbital blood samples were collected respectively at the 1st,3rd and 28 th day after administration.The heart,lung,liver,kidney,spleen and other organs tissue samples were collected for pathological section,HE staining.The histopathological changes were investigated,and the routine blood test was proceeded.Results The WBC of LCWE group showed a trend of rising at the 1st,3rd and 28 th day after administration.PLT and MPV of LCWE group increased at the 3rd day after administration,which returned to normal levels at the 28 th day after administration.The pathological section showed the blood vessel walls of heart tissue enlargement,surrounded and infiltrated by inflammatory cells infiltration,the atheromatous plaque in blood vessels occasionally.Conclusion The study established mice model of Kawasaki disease successfully.
出处
《国际检验医学杂志》
CAS
2014年第21期2867-2868,2871,共3页
International Journal of Laboratory Medicine
基金
广东省自然科学基金资助项目(S2013010012520)
关键词
川崎病
干酪乳杆菌
细胞壁
小鼠
Kawasaki disease
Lactobacillus casei
cell wall
mice
