摘要
目的 研究皮肤组织原位记忆T淋巴细胞在银屑病发病过程中的作用.方法 收集32例进行期斑块状银屑病患者的临床资料,采集每例患者的皮损和非皮损组织、9例患者皮损消退后皮肤标本;10例健康人皮肤为对照.采用免疫组化法检测组织原位记忆T淋巴细胞的特征性表面标志CD69和CD103,分析组织原位记忆T淋巴细胞在银屑病发病不同时期的情况.两组免疫组化结果进行t检验.结果 32例进行期银屑病患者皮损和非皮损每高倍视野中CD69＋CD103＋T淋巴细胞数量分别为11.34±7.60和2.72±4.20,皮损区明显高于非皮损区（t=8.46,P＜0.01）;其中9例患者皮损消退前后每高倍视野中CD69＋CD103＋T淋巴细胞数量分别为14.33±2.21和12.00±4.58,皮损消退前后比较差异无统计学意义（t=1.98,P=0.08）;健康对照组为1.70±2.98,与银屑病患者非皮损区比较,差异无统计学意义（t=0.71,P＞0.05）.结论 银屑病患者皮肤中原位记忆T淋巴细胞可能在皮损的形成和复发过程中起作用.
Objective To investigate the role of tissue-resident memory T lymphocytes in the pathogenesis of psoriasis.Methods Clinical information was collected from 32 patients with progressive plaque psoriasis.Tissue specimens were obtained from both lesional and nonlesional psoriatic skin of all the patients,as well as from faded lesions in 9 of these patients.Tissue specimens from the normal skin of 10 healthy individuals served as the controls.Immunohistochemical staining was performed to detect the two characteristic surface markers CD69 and CDI03 on tissue-resident memory T lymphocytes and to analyze the status of these T lymphocytes at different stages of psoriasis.The results of immunohistochemical staining were compared by t test.Results The mean number of CD69＋CD103＋ T lymphocytes per high-power field was significantly higher in lesional skin than in nonlesional skin of the 32 patients （11.34 ± 7.60 vs.2.72 ± 4.20,t =8.46,P 〈 0.01）,but similar between psoriatic lesions in the 9 patients before and after subsidence （14.33 ± 2.21 vs.12.00 ± 4.58,t =1.98,P =0.08）.There was no significant difference in the mean number of CD69＋CD103＋ T lymphocytes between nonlesional psoriatic skin and normal control skin （2.72 ± 4.20 vs.1.70 ± 2.98,t =0.71,P 〉 0.05）.Conclusion Tissue-resident memory T lymphocytes may play a role in the formation and recurrence of psoriatic lesions in patients.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2014年第11期800-802,共3页
Chinese Journal of Dermatology
