摘要
目的 探讨人脑胶质瘤中层粘连蛋白 (LN)、纤维连接蛋白 (FN)及突变型p5 3基因蛋白的免疫组织化学与肿瘤侵袭微生态系统 (tumorousinvasionmicroecosystem ,TIMES)的相关性。 方法 利用透射电镜观察TIMES中微血管特征和免疫组织化学SP法评价LN、FN、p5 3的表达情况 ,并作比较分析。 结果 1 脑胶质瘤微血管基底膜 (basemembrane ,BM)连续 ,多数呈局限性或广泛性增厚 ,并与LN、FN染色结果相一致 ,也与p5 3染色与否有关 ,p5 3阳性染色者BM增厚较p5 3阴性者明显。 2 LN、FN在所有胶质瘤微血管BM及内皮细胞上呈阳性染色 ,恶性程度越高 ,BM上LN、FN染色阳性越强 ,血管管壁越厚 (P <0 0 1和P <0 0 5 ) ,而瘤细胞未见染色。LN在脑内转移瘤BM和内皮细胞上却未见染色 ,但可见散在瘤细胞浆膜染色 ;FN在脑内转移瘤上的染色则与脑胶质瘤相类似。 3 45例胶质瘤中p5 3阳性染色 2 1例 ,其p5 3阳性染色与否也与BM上LN、FN染色结果存在密切正相关 (P <0 0 1)。p5 3阳性染色率在脑内转移瘤和恶性胶质瘤中无统计学差别 (P >0 0 5 )。 结论 脑微血管内皮细胞上LN、FN的过分表达可能是脑胶质瘤TIMES中BM形态学增厚的原因之一 ,p5 3对TIMES的影响也与微血管的内皮细胞功能状态有关。血管内皮细胞可能是TIMES的调控?
Objective To explore the relationship between immunohistochemistry of LN,FN,p53 and TIMES in human brain glioma(BG). Methods Transmission electronic microscope(TEM) and immunohistochemistry were used to investigate the morphological characteristics of micrangiums in BG and the expression of LN,FN,p53 in BG and intracranial metastatic tumors. Results 1.It was found that base laminas beneath endothelial cell with locally or extensively thickened were intact and continuous in BG.The increasing thickness of BM was consistent with the staining of LN and FN and related to p53 immunostaining.BM of p53-protein positive cases grew thicker than that of p53-protein negative ones.2.Micrangiums BM in all BG were positive for LN and FN.The more malignant the BG was,the stronger the LN and FN staining became and the thicker the blood vessel walls grew(P<0.01,P<0.05,respectively).Cytoplasms instead of nuclei of endothelial cells were also positive for LN and FN and there was no staining in glioma cells.There was no staining in BM or endothelial cells in intracranial metastatic tumors except cellular membranes of scattered glioma cells.Otherwise,the staining of FN in intracranial metastatic tumors was similar to that of FN in BG.3.21/45 cases showed positive for p53-protein.The positive staining of p53 was significantly correlated with the results of LN and FN immunostaining(P<0.01).The difference of p53 immunostaining between intracranial metastatic tumors and high malignant glioma was not statistically significant(P>0.05).Conclusion One of the reasons that BMs in TIMES in BG thicken may be the over expression of LN and FN of brain micrangium endothelial cells.Also,the influence of p53 on TIMES is associated with the functional state of endothelial cells.Micrangiums endothelial cells may be play a role in regulating TIMES.
出处
《解剖学报》
CAS
CSCD
北大核心
2002年第4期360-365,共6页
Acta Anatomica Sinica
基金
福建省科技厅三项费资助项目 ( 98Z171)
