摘要
目的：建立同时测定镇惊泻火颗粒中芒果苷、芦荟大黄素、大黄素和大黄酚含量的方法。方法：采用高效液相色谱法。色谱柱为Capcell Pak C18（250 mm×4.6 mm,5μm）,流动相为乙腈-0.1%甲酸水溶液（梯度洗脱）,流速为1.0 ml/min,检测波长为254nm,柱温为20℃结果：芒果苷、芦荟大黄素、大黄素和大黄酚的质量浓度分别在10.180-101.800、2.016-20.160、1.072-10.720、1.024-10.240μg/ml范围内与各自峰面积积分值呈良好的线性关系（r分别为0.9998、0.9996、0.9995、0.9996）;精密度、稳定性、重复性试验的RSD≤1.4%（n均为6）;平均加样回收率分别为98.05%、97.39%、97.91%和98.93%,RSD分别为1.3%、1.5%、1.2%和1.4%（n均为6）。结论：该方法准确、稳定、重复性好,可用于镇惊泻火颗粒的质量控制。
OBJECTIVE：To establish a method for the content determination of mangiferin,aloe-emodin,emodin,chrysophanol in Zhenjing xiehuo granule. METHODS：HPLC method was adopted. The determination was performed on Capcell Pak C18（250 mm×4.6 mm,5 μm）column with mobile phase consisted of acetonitrile-0.1% formic acid（gradient elution）at the flow rate of 1.0 ml/min. The detection wavelength was set at 254 nm. The column temperature was 20 ℃ RESULTS：The linear ranges were10.180-101.800 μg/ml for mangiferin（r=0.999 8）,2.016-20.160 μg/ml for aloe-emodin（r=0.999 6）,1.072-10.720 μg/ml for emodin（r=0.999 5）and 1.024-10.240 μg/ml for chrysophanol（r=0.999 6）,respectively. RSD of precision,stability and reproducibility tests were all lower than 1.4%（n=6）. The average recoveries were 98.05%（RSD=1.3%,n=6）,97.39%（RSD=1.3%,n=6）,97.91%（RSD=1.4%,n=6）and 98.93%（RSD=1.5%,n=6）,respectively. CONCLUSIONS：The method is accurate,reliable,reproducible and specific,and is suitable for quality control of Zhenjing xiehuo granules.
出处
《中国药房》
CAS
CSCD
2014年第39期3687-3689,共3页
China Pharmacy
基金
上海市卫生局中药新药及院内制剂研发项目(No.2010Y013A)
