摘要
目的探讨IE mRNA和pp67 mRNA在更昔洛韦治疗后的人巨细胞病毒(HCMV)感染患儿中的表达情况。方法在接受更昔洛韦治疗前,治疗2周和停药4周时检测HCMV患儿总胆红素(TBIL)、直接胆红素(DBIL);丙氨酸转氨酶(ALT)、碱性磷酸酶(ALP)及荧光定量PCR的方法检测HCMV-DNA拷贝数,IE mRNA和pp67 mRNA的表达采用实时荧光定量PCR法进行检测。结果更昔洛韦治疗2周后,患儿肝功能及血HCMV-DNA均较治疗前降低,停药4周后TBIL、DBIL、ALT、ALP及HCMV-DNA拷贝数均较治疗前显著降低,但是与治疗2周比较差异无统计学意义。治疗后2周,仍有12例患儿血液中表达pp67 mRNA,表达量显著低于治疗前的pp67mRNA表达量,但检测不到患儿表达IE mRNA。停药4周,pp67 mRNA阳性表达的患儿数量显著低于治疗2周的患儿数量,有3例患儿表达微弱的IE mRNA。2个基因的mRNA表达量与治疗2周比较差异无统计学意义。结论在更昔洛韦治愈患儿,HCMV-DNA检测呈现阴性时,应再进行病毒mRNA检测,对RNA表达阳性的患儿,应该加强随访与复查,预防病毒复发。
Objective To study the IE and pp67 mRNA expressions in infants infected human cytomegalovirus （HCMV） after ganciclovir treatment. Methods The values of total bilirubin （TBIL）, direct bilirubin （DBIL）, alanine amino-transferase （ALT） and alkaline phosphatase （ALP） were detected in patients with HCMV before treatment, 2 weeks and 4 weeks after treatment. The HCMV-DNA was detected by quantitative PCR. The expression levels of IE mRNA and pp 67 mRNA were detected by real-time fluorescent quantitative PCR. Results HCMV DNA stopped copying and the infected in-fants were cured following the traditional criterion, however, RNA expression was still tested in part of these infants. Conclusion It should be identified for mRNA expression when HCMV-DNA copies were hardly identified in these infants to prevent from relapse.
出处
《天津医药》
CAS
北大核心
2014年第9期940-942,共3页
Tianjin Medical Journal
