摘要
[目的]建立一种同时测定葡萄中多效唑和烯效唑残留的气相色谱（GC-ECD）的分析方法。[方法]样品经乙腈提取,氯化钠分离处理、弗罗里硅土固相萃取柱净化,丙酮-正己烷（体积比15颐85）洗脱,浓缩液通过毛细管气相色谱柱分离,用ECD检测器测定。[结果]农药的添加水平在0.02～0.10 mg/kg范围时,2种农药的添加回收率在87.3%～104.8%之间,相对标准偏差为4.2%～10.7%,多效唑的定量限为0.02 mg/kg;烯效唑定量限为0.006 mg/kg。[结论]该方法灵敏、准确,能够满足葡萄中多效唑和烯效唑残留检测的要求稳定、可靠,适用于葡萄中多效唑和烯效唑的同时检测。
[Aims] Simultaneous determination of paclobutrazol and uniconazole residues in grape by GC/ECD was introduced. [Methods] The samples were extracted with acetonitrile, and the extract were separated by sodium chloride. After cleanup via florisil column with elution solvents of acetone and hexane （15：85, by vol）, the concentrated solution was separated by capillary gas chromatography column. Finally, the samples were determined by ECD. [Results] When the spiked levels were 0.02, 0.05 and 0.10 mg/kg, the average recoveries of pesticides were 87.3-104.8%, with relative standard deviations （RSDs） in the range of 4.2-10.7%. The limit of quantitation （LOQ） of paclobutrazol and uniconazole were 0.02 and 0.006 mg/kg, respectively. [Conclusions] The method is sensitive, accurate, and satisfied for detection of paclobutrazol and uniconazole residues in grape.
出处
《农药》
CAS
CSCD
北大核心
2014年第10期736-738,755,共4页
Agrochemicals
基金
2014年国家农产品质量安全风险评估计划(2014FP02)
关键词
气相色谱法
葡萄
多效唑
烯效唑
残留
gas chromatography
grape
paclobutrazol
uniconazole
residue
