摘要
目的探讨全反式维甲酸(ATRA)对淀粉样蛋白(Aβ)42诱导的星形胶质细胞凋亡的影响及其作用机制。方法原代培养星形胶质细胞,分为3组,正常组加入200μL正常的DMEM/F12培养基,对照组加入200μL不含ATRA而含有2μg/mL Aβ42培养基,实验组分别加入200μL含有0.01、0.1、1、10μmol/L ATRA且同时含有2μg/mL Aβ42培养基。MTT法检测细胞存活率,qRT-PCR、Western blot法分别检测载脂蛋白(Apo)E和BACE1的mRNA、蛋白表达变化。结果各实验组星形胶质细胞凋亡作用不同,其中实验组0.1、1μmol/L的星形胶质细胞凋亡率较低,差异有统计学意义(P<0.05)。与对照组比较,实验组0.1、1μmol/L中ApoE的mRNA、蛋白表达上调,差异有统计学意义(P<0.01),BACE1 mRNA、蛋白表达无明显变化。结论 ATRA可以上调ApoE表达,抑制Aβ42诱导的星形胶质细胞凋亡。低浓度ATRA对星形胶质细胞有保护作用。
Objective To investigate the inhibitive effects of all- trans retinoic acid（ ATRA） on amyloid β-peptide 42（ Aβ42）- induced apoptosis of astrocytes. Methods The MTT assay was applied for detection of cell survival,while qRT- PCR and Western blot were applied for assessment of ApoE and BACE1 expression. Astrocytes were cultured with different concentrations of ATRA for induction of apoptosis,while DMEM /F12 medium alone was used as control. Results The effects of the different concentrations of ATRA on protecting against the Aβ42- induced apoptosis of astrocytes were different,as ATRA in 0. 1 and 1 μmol /L significantly inhibited the apoptosis induced by Aβ42（ P〈 0. 05）. Compared with control group,the expression of ApoE was significantly up- regulated by ATRA in 0. 1 and 1μmol/L（ P 〈0. 01）,while there was no significant difference in the expression of BACE1. Conclusion ATRA can up- regulate the expression of ApoE,prohibit the astrocytes apoptosis induced by Aβ42. Low concentration ATRA have protective effect on astrocytes.
出处
《广东医学》
CAS
CSCD
北大核心
2014年第17期2649-2651,共3页
Guangdong Medical Journal
基金
国家自然科学基金资助项目(编号:81100945)
中国博士后科学基金面上项目(编号:201150M1577)
中国博士后科学基金特别资助项目(编号:2012T50900)
