摘要
目的探讨镉对培养大鼠睾丸支持细胞相关蛋白表达、p38MAPK磷酸化及超微结构的影响和黄芪甲苷的保护效应。方法对照组、镉(50mol/L)处理组、镉(50mol/L)加黄芪甲苷(10mg/L)组的培养支持细胞用于超微结构观察、波形蛋白、E-钙粘连蛋白/-环连蛋白免疫组织化学及磷酸化p38MAPK检测。结果镉处理组支持细胞线粒体内室肿胀,脂滴堆积,内质网扩张和(或)空泡化,髓样结构形成,少许支持细胞出现凋亡,镉加黄芪甲苷组支持细胞超微结构改变较镉处理组轻;免疫组织化学显示镉处理组波形蛋白、E-钙粘连蛋白及-环连蛋白阳性产物较对照组明显减弱(P<0.05),镉加黄芪甲苷组阳性产物虽较对照组减少但明显高于镉处理组(P<0.05);镉处理组支持细胞内磷酸化P38MAPK阳性产物表达量较对照组明显增强,且有向细胞核移位趋势,镉加黄芪甲苷组阳性产物表达量明显少于镉处理组(P<0.05)。结论镉致大鼠睾丸支持细胞超微结构损伤、细胞骨架蛋白及粘连蛋白破坏并增强P38MAPK磷酸化;黄芪甲苷可拮抗镉的毒性,其保护效应可能与减少P38MAPK的磷酸化等有关。
Objective To investigate the toxic effect of cadmium （Cd） on the ultrastructure ,expression of related protein and the signal molecule phosphrylated P38 mitogen-actived protein kinase（P-P38MAPK） of primary cultured rat sertoli cell（Sc） ,and the protective effect of astragaloside （A ） on it .Methods The primary cultured rat Sc were divided into the control group ,Cd （50 mol/L）group and Cd（50 mol/L） plus A（10 mg/L） group ,they were used for the electron microscope observation and the im-munohistochemistry detection of vimentin ,E-cadherin ,-catenin and P-P38MAPK .Results The Sc ultrastructural changes included that the swelled mitochondria ,abundant lipid droplets and dilated endoplasmic reticulum were found in the Cd group .Further ,apop-tosis occurred in some Sc .However these ultrastructure changes above mentioned were slighter in the Cd plus A group ;the immu-nohistochemistry showed that the positive products of vimentin ,E-cadherin and-catenin were obviously decreased in the Cd group （P〈0 .05） ,and those in the Cd plus A group were higher compared with the Cd group（P〈0 .05）;the expression of P-P38MAPK in cytoplasm was increased in the Cd group ,and showed the trend to move from cytoplasm to nucleus ,meanwhile ,the positive prod-ucts expression in the Cd plus A group was lower than that in the Cd group （P〈0 .05） .Conclusion Cadmium can cause the injury of the Sc ultrastructure ,damage of cytoskeletal protein and fibronectin ,and increase of P-P38MAPK level ;astragaloside can antago-nize the toxicity of cadmium on Sc ,the protective effect maybe related with the decrease of P-P38MAPK in Sc .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第27期3592-3595,共4页
Chongqing medicine
关键词
镉
黄芪甲苷
支持细胞
相关蛋白表达
磷酸化P38M APK
cadmium
astragaloside
phosphorylated P38MAPK
sertoli cell
expression of ralated protein
