摘要
目的:建立肾康胶囊中大黄酚、大黄素的含量测定方法。方法:采用HPLC法测定肾康胶囊中大黄酚、大黄素含量。色谱条件为:Thermo C18色谱柱(4.6mm×250mm,5μm);以甲醇-0.1%磷酸溶液(80∶20)为流动相;流速为1.0mL·min-1;检测波长为254nm;柱温为30℃。结果:大黄酚在0.02~0.64μg范围内呈良好的线性关系(r=0.9996),平均回收率为97.18%,RSD为1.13%(n=6);大黄素在0.016~0.512μg范围内呈良好的线性关系(r=0.9994),平均回收率为96.77%,RSD为1.62%(n=6)。结论:该方法准确、重现性好、专属性强,可为肾康胶囊的质量控制提供参考。
Objective：To establish a determination method of Emodin andchrysophanol of Shenkang Capsules. Methods：To deter- mine chrysophanol and emodin of Shenkang capsule by HPLC. Thermo Cls was used with Methanol -0.1% phosphoric acid （80 ： 20） as the mobile phase, The flow rate was 1.0 mL/min, detective wavelength was 254 nm, column temperature was 30 ℃. Re- sults：Chrysophanol showed a good linear relationship in the range of 0.02~0. 64μg （r= 0. 9996） The average recovery（n= 6） 97.18% ,RSD 为 1.13%. and Emodin showed a good linear relationship in the range of 0. 016μg^0. 512μg （r=0. 9994）. The aver- age recovery（n= 6）96.77%, RSD 为 1.62 %. Conclusion：The method was accurate, good reproducibility, specificity, and can con- trol the quality of this preparation.
出处
《亚太传统医药》
2014年第17期39-40,共2页
Asia-Pacific Traditional Medicine
