摘要
目的 观察老年Wistar大鼠自发性肿瘤发生情况、检测血液学及血清生化指标变化.方法 SPF级Wistar大鼠饲养2年后处死,测定动物外周血及血清生化指标,在试验过程中观察动物的一般状态,记录自发性肿瘤发生情况.结果 Wistar大鼠饲养于SPF设施,共观察104周,皮肤和皮下组织、脂肪组织系统肿瘤最为多见,其次为神经系统、生殖系统.消化系统肿瘤仅发生于雄性大鼠.白细胞、红细胞平均体积、碱性磷酸酶、白蛋白、葡萄糖、总胆固醇和肌酸激酶性别间差别显著.结论 建立老年Wistar大鼠的相关正常值参考范围,为建立和开展新药的啮齿类动物致癌性试验评价提供基础数据.
Objective To measure spontaneous tumorigenesis, hematological, and serum biochemical parameters of Wistar rats feeding for two years. Methods Wistar rats feeding for two years were killed to determinate peripheral blood and serum biochemical parameters, and spontaneous tumorigenesis was also investigated. Results Wistar rats were raised in GLP laboratory for two years, the cancer of skin, hypodermis and adipose tissue was widespread, tumorigenesis of nervous or genital system was also observed, and tumorigenesis of digestive system was found in male rats. Compared with female rats, parameters of white blood cells （WBC）, mean corpuscular volume （MCV）, alkaline phosphatase （ALP）, albumin （Alb）, glucose （Glu）, total cholesterol （CHO）, creatine kinase （CK） were changed obvi- ously in male rats.Conclusion Normal reference range of Wistar rats feeding for two years was set up in order to evaluate rodent animals carcinogenicity for new drug.
出处
《实验动物与比较医学》
CAS
2014年第4期324-329,共6页
Laboratory Animal and Comparative Medicine
关键词
WISTAR大鼠
自发性肿瘤
血常规
血清生化
Wistar rats
spontaneous tumorigenesis
hematological parameters
biochemical parameters
