摘要
本研究通过免疫组织化学和组织化学法检测喉鳞状细胞癌(LSCC)中的血管生成拟态(VM)和微血管密度(MVD)情况,并研究它们与半乳糖凝集素-3(Gal-3)以及LSCC患者临床病理因素之间的相互关系。在正常喉组织中未见VM现象,而LSCC组织中VM阳性率为33.7%,两组之间差异有统计学意义(P<0.01)。VM情况以及MVD计数在LSCC患者的不同年龄、性别、肿瘤位置等亚组之间差异无统计学意义(P>0.05),在肿瘤不同分化程度、临床病理分期以及淋巴结转移状态亚组之间差异有统计学意义(P<0.05)。LSCC组织中VM阳性率、MVD计数与Gal-3蛋白表达两两之间均为正相关关系(P<0.05)。研究结果提示Gal-3可能参与了LSCC的发生、血管生成以及VM形成,且Gal-3、血管生成以及VM形成均促进LSCC的发展、侵袭及转移。
Abstract： The present paper aims to investigate whether or not vasculogenic mimicry （VM） exists in laryngeal squamous cell carcinoma （LSCC）, and to elucidate its relationship to microvessel density （MVD）, galectin-3 （Gal-3） expressionb and clinicopathological factors of patients with LSCC. VM, score of MVD and expression of Gal-3 protein were detected by immunohistochemistry and histochemistry in 83 specimens of LSCC tissue and 20 specimens of normal laryngeal tissue. The positive rate of VM in normal laryngeal tissues was 0%, and was 33.7% in LSCC tissues. There was a significant difference between the two groups （P〈0.01）. VM or MVD was significantly related to differentiation, pTNM stages and lymph node metastasis of LSCC （P〈0.05）, but not to age, gender and tumor site （P〉0.05）. And there was a positive correlation between every two of VM, score of MVD, and Gal-3 protein （P〈 0.05）. The results suggest that expression of Gal-3 protein may be related to the initiation, angiogenesis and VM formation in LSCC; And VM, angiogenesis and Gal-3 protein may be involved in the development, invasion and metastasis of LSCC.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2014年第4期865-869,共5页
Journal of Biomedical Engineering
