摘要
目的观察参附注射液对大鼠肢体缺血再灌注损伤后肝功能、血红素加氧酶-1(HO-1)表达的影响,并对其保护机制做一初步探讨。方法 64只清洁级SD雄性大鼠,用随机数字表法随机分为4组,每组16只,分别为假手术组、缺血再灌注组、参附干预组、参附+锌原卟啉Ⅸ(Znpp)干预组。假手术组:大鼠麻醉后仅分离不夹闭股动脉,分离血管前10 min以7.5 mL/kg腹腔注射生理盐水;缺血再灌注组:夹闭股动脉前10 min以7.5 mL/kg腹腔注射生理盐水,夹闭股动脉缺血3 h,再灌注4 h;参附干预组:夹闭股动脉前10 min以7.5 mL/kg腹腔注射参附注射液,夹闭股动脉缺血3 h,再灌注4 h;参附+Znpp干预组:术前30 min腹腔注射Znpp 5 mg/kg,余同参附干预组。再灌注完毕后取材,取外周静脉血测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)含量;取肝组织测定肝组织中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性;采用免疫组织化学法测定肝脏组织中HO-1蛋白表达;光镜下观察肝脏病理学改变。结果 1与假手术组比较,各肢体缺血再灌注造模组MDA含量均明显升高(P<0.05),SOD活性明显降低(除参附干预组外,P<0.05),GPT、GOT含量均明显升高(P<0.05),HO-1蛋白表达明显升高(P<0.05)。2与缺血再灌注组比较,参附干预组MDA含量明显降低(P<0.05),SOD活性明显升高(P<0.05),血清GPT、GOT含量明显降低(P<0.05),肝组织中HO-1蛋白表达升高(P<0.05)。3与参附干预组比较,参附+Znpp干预组MDA含量明显升高(P<0.05),SOD活性明显降低(P<0.05),血清GPT、GOT含量明显升高(P<0.05),而肝组织HO-1蛋白表达差异无统计学意义(P>0.05)。结论肢体缺血再灌注可造成肝脏功能损伤,给予参附注射液预处理可以减轻肝脏损害程度,这种保护作用可能与参附注射液预处理上调HO-1蛋白在肝组织中的表达、抑制氧自由基生成有关。
Objective To investigate the protective effect of Shenfu injection on liver injury in rats with hind limb ischemia-reperfusion and its mechanism. Methods Sixty-four male rats were randomly divided into sham operation group, ischemia-reperfusion group, Shenfu group [ Shenfu injection 7. 5 mL/kg injection of peritoneal （ip）, given 10 min before ischemia-reperfusionJ, Shenfu＋Znpp group （Shenfu injection 7. 5 mL/kg＋Znpp 5 mg/kg ip, given 10 rain before ischemia-reperfusion）, 16 rats in each group. The rat model of hind limb ischemia-reperfusion injury was reproduced by occluding the hind limb artery of the rats for 3 h and subsequent reperfusing for 4 h. The liver tissues were gathered for malondialdehyde （MDA） and superoxide dismutase （SOD） determination. The expression of hemeoxygenase 1 （HO-1） protein in the liver tissues was detected by immunohistochemistry. The pathological changes of liver were observed under the light microscope. lne changes oI serum gmtamate-py~ uvat~ glutamine oxaloacetic transaminase （GOT） were observed respectively. Results （~） Compared with the sham operation group, the contents of MDA, GPT, GOT, and the expression of HO-1 protein were markedly increased in the ischemia-reperfusion group, Shenfu group, and Shenfu ＋ Znpp group （P〈 0.05）, the activities of SOD were markedly decreased in the ischemia-reperfusion group and Shenfu ＋ Znpp group （P〈 0.05）. ~）Compared with the ischemia-reperfusion group, the contents of MDA, serum GPT, GOT, and the expression of HO-1 protein were markedly decreased, the activity of SOD was markedly increased in the Shenfu group O~〈 0. 05）. ~ Compared with the Shenfu group, the contents of MDA, GPT, GOT were markedly increased, the activity of SOD was markedly decreased in the Shenfu ＋ Znpp group （P〈 0. 05）. Unde ther light microscope, the pathological changes induced by ischemia-reperfusion were significantly attenuated by the Shenfu injection in the Shenfu group and were reversed by the Znpp in the Shenfu ＋ Znpp group. Conclusion Shenfu injection inhibits liver tissue injury during hind limb ischemia-reperfusion, this protective effect might be partly through induction of HO-1.
出处
《中国普外基础与临床杂志》
CAS
2014年第8期960-964,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
兰州市科技计划项目(编号:2012-1-49)~~
